Cell freezing media at a glance
abm offers two ready-to-use freezing media so labs can select the formulation that fits their established cell culture workflow.
- CryoGuard™ Freezing Media: 50 mL, serum-free, xeno-free, ready-to-use, $99 USD.
- Cryopreservation Medium: 100 mL, complete serum-containing medium with 10% DMSO, $445 USD.
- First-time buyer promo: Save 15% on CryoGuard™ Freezing Media with code CRYOG2026.
Products
Choose from abm’s two ready-to-use freezing media. Each product card below includes the formulation, format, price, and direct product action.
CryoGuard™ Freezing Media
Cat. No. TM078 • 50 mLCryoGuard™ Freezing Media
CryoGuard™ Freezing Media is abm’s serum-free, xeno-free cryopreservation medium with validation data for post-thaw and day 1 recovery across mammalian cell lines.
Key Benefits
Every feature of CryoGuard™ is built around practical cryopreservation needs: reproducibility, simplicity, cell recovery, and value.
No FBS Lot Variability
Replace homemade DMSO + serum freezing media with a serum-free, xeno-free formulation designed to reduce batch-to-batch variability.
Strong Post-Thaw Viability
CryoGuard™ was tested head-to-head against 70% complete medium + 20% FBS + 10% DMSO controls across multiple cell lines.
No Mixing, No Extra Preparation
Skip manual formulation steps and reduce contamination risk with a convenient medium that is ready when your cells are.
Performance Without the Premium Price
At $99 USD per 50 mL, CryoGuard™ offers a practical alternative to higher-cost commercial cryopreservation reagents.
CryoGuard™ Product Details
Defined Research Formula
Built to reduce variability in routine cryopreservation.
| Formulation | Serum-Free, Xeno-Free |
| Sterility | Tested |
| Validated Lines | 50+ cell lines |
| Growth Type | Adherent & Suspension |
Ready-to-Use Workflow
Designed for easy integration into common freezing protocols.
| Long-Term | -20°C |
| Working Stock | 2-8°C |
| Format | Ready-to-use |
| Freeze Method | Controlled-rate or IPA |
CryoGuard™ Performance Data
CryoGuard™ validation included immediate post-thaw and day 1 recovery measurements across multiple mammalian cell lines. Human iPSCs were benchmarked against a commercial ES reagent.
CryoGuard™ vs. DMSO/FBS Control
Grouped comparison across five validated mammalian cell lines.
24 h Post-Thaw Recovery
Day 1 viability across adherent and suspension lines.
CryoGuard™ vs. Commercial ES
Human iPSC post-thaw and day 1 viability benchmarked against a commercial ES reagent.
| Cell Line | Cat. No. | Growth Type | CryoGuard™ Post-Thaw | CryoGuard™ Day 1 | Comparator |
|---|---|---|---|---|---|
| OVUM 50.2 | N/A | Suspension | 95% | DMSO/FBS: 73% / 95% | |
| 293-S | T8030 | Suspension | 95% | DMSO/FBS: 78% / 95% | |
| 293T | LV010 | Adherent | 95% | DMSO/FBS: 99% / 95% | |
| Dermal Papilla (O Clone 1) | T0875 | Adherent | 95% | DMSO/FBS: 100% / 95% | |
| CHO-K1 | N/A | Adherent | 90% | DMSO/FBS: 98% / 100% | |
| Human iPSC | N/A | Adherent | 90% | Commercial ES: 97% / 90% |
Human iPSCs were tested against a commercially available embryonic stem cell cryopreservation reagent rather than the standard DMSO/FBS control to reflect serum-free pluripotent cell culture requirements.
CryoGuard™ Validation Study Comparison
The validation study compared CryoGuard™ against a conventional in-house DMSO/FBS freezing medium.
DMSO / FBS Medium
- 70% complete medium + 20% FBS + 10% DMSO
- Contains animal serum
- Requires in-house preparation
- Subject to FBS lot variability
CryoGuard™ Freezing Media
- Defined, serum-free, xeno-free formulation
- No animal serum components
- Ready-to-use format
- Designed for consistent freezing workflows
Applications
Use these media for common mammalian cell freezing and recovery workflows.
Routine Cell Banking
Freeze working stocks and backup cell banks with ready-to-use media.
Adherent Cell Lines
Support cryopreservation of commonly used adherent mammalian lines.
Suspension Cell Lines
Use across suspension workflows such as OVUM 50.2 and 293-S cultures.
Stem Cell Workflows
Choose serum-free workflows when appropriate for pluripotent cells.
Cell Freezing Media FAQs
Quick answers about formulation, evaluation, and ordering.
Which product is serum-free?
CryoGuard™ Freezing Media is the serum-free, xeno-free option. Cryopreservation Medium contains serum and 10% DMSO.
When should I choose Cryopreservation Medium?
Choose Cryopreservation Medium when your lab prefers a complete serum-containing cryopreservation medium for classical mammalian cell culture workflows.
How was CryoGuard™ evaluated?
CryoGuard™ Freezing Media was evaluated at immediate post-thaw and 24-hour recovery timepoints using comparator freezing media.
What is the first-time buyer promo code?
First-time buyers can use promo code CRYOG2026 for 15% off CryoGuard™ Freezing Media, subject to promo eligibility and checkout terms.