Stable Cell Line Generation Service
abm offers a high-quality, cost-effective service designed to express your gene of interest quickly and efficiently. With a fast turnaround time and customized support, we simplify your research and help you meet your project goals with ease.
Leveraging our extensive library of gene expression vectors and deep expertise in cell culture, we tailor each project to your specific needs. Our streamlined process includes:
- Lentiviral vector construction
- Lentivirus packaging and transduction
- Clone selection
- Gene expression validation via qPCR
Have additional requirements? Let us know—we’re here to support your unique research needs every step of the way.
Service Details
| OUR BASIC PACKAGE (C056) INCLUDES | DELIVERABLES |
|---|---|
|
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| Add-On Service | Unit | Cat. No. | Price |
|---|---|---|---|
| Gene Expression Assay Service^ | 1 Marker | C138 | $165.00 |
| Additional Clones^^^ | 1 Clone | C160 | $350.00 |
| Additional Vials of Delivered Cells^^^ | 1 Vial | C144 | $300.00 |
| Tag Validation via Western Blot (Up to 10 Clones Tested)# | 1 Service | C191 | $400.00 |
| Western Blot Validation Service## | 1 Service | C145 | $975.00 |
|
Cell Line Authentication: (For other species, please inquire) |
1 Service | C287 | $195.00 |
# Service only applicable if gene of interest has an HA tag. abm will provide HA tag antibody.
## Please provide validated antibody and positive control
* Cells provided by customers must be mycoplasma-negative at the time of submission
^^^ Additional media or supplements may be required. For for-profit organizations and corporations, the price is 1.5 times the listing price.
Additional Info
Follow these steps to order a Stable Cell Line Generation Service (quick view):
-
To obtain a quote, please fill out the service questionnaire and submit to quotes@abmgood.com.
- Place an order and specify the quote number during order placement. Note that samples received without an order confirmation number indicated will be disposed immediately upon receipt to ensure that all customer information is held in strict confidence.
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Once you have received an order confirmation number, please set up shipment and submit:
- Cells: At least 2x106 cells/cell line along with passage number. You may also select any of the cell lines in our catalog.
- Complete Cell Culture Protocol
- What Antibiotic Resistance Markers Do Your Cells Have? Our vectors have puromycin resistance by default.
- Media: All necessary media components, including any applicable FBS, growth factors, etc. required for culturing (abm will only provide DMEM and RPMI). Please submit all components of the complete media individually. Also include instructions for making the complete media. Volume requirement: ≥1 L of complete media.
- Flasks: ≥5x coated 6-well plates & 10x T25 flasks if the cells require specially-treated vessels.
- Standard Verification Method: RT-PCR; please specify prior to order placement if other another option is preferred.
- Morphology of Cells: Please supply an image of the cells growing in culture to depict 'original' cell morphology prior to shipment.
- abm will generate the desired stable cell line and perform QC.
Customer Review:
"abm has always been very helpful with technical assistance in any of our questions and concerns. I also consider the price for the services provided as very competitive, as other companies charge a multiple of abm's price."
Dr. Cameron Moshfegh, ETH ZurichRelated Products
Citations
| 01 | Zhang, J et al. "The construction and proliferative effects of a lentiviral vector capable of stably overexpressing SPINK1 gene in human pancreatic cancer AsPC-1 cell line." Tumour Biol 37(5):5847-55 (2016).DOI: 10.1007/s13277-015-4405-z PubMed: 26586397 |