Cell Immortalization Reagents
Immortalized cells are highly preferred over primary cells due to their ability to provide unlimited, consistent access to the same cell line. This eliminates the issues of batch-to-batch variability and the high costs associated with primary cell use.
As a pioneer in cell immortalization technology, abm offers:
- A comprehensive library of immortalized cell lines for human, mouse, and other key research model organisms.
- Industry-leading immortalization reagents for researchers who wish to immortalize their own primary cells.
Our selection features the widest range of reagents and the highest quality standards—areas where competitors simply can't compare. Whether you're seeking ready-to-use cell lines or advanced tools to immortalize your own, abm has you covered.
Recombinant SV40T Virus
| SV40 T Antigen: Proven, Robust, and Versatile |
|
SV40 large T antigen is a gold-standard for efficiently immortalizing primary mammalian cells. It enables continuous proliferation through multiple mechanisms:
• Inactivates p53 and Rb, bypassing cell cycle checkpoints • Activates E2F, driving cells into S phase • Reactivates telomerase, preventing telomere shortening • Stabilizes replication machinery, supporting sustained in vitro growth Whether you're developing epithelial, kidney, or respiratory models, SV40 T antigen ensures robust performance and reliable expansion. |
| Product Name | Cat. No. | Vector Map | Promoter | Marker | Price |
|---|---|---|---|---|---|
| Lentivirus | |||||
| Lenti-SV40T (Puro) Virus | LV613 | View | CMV | Puro | Inquire |
| Lenti-SV40T (Neo) Virus | LV660 | View | CMV | Neo | Inquire |
| Lenti-SV40T Virus | LV665 | View | CMV | Inquire | |
| Lenti-SV40Tt Virus | LV614 | View | CMV | Puro | Inquire |
| Lenti-SV40 Virus | LV612 | View | CMV | Inquire | |
| Lenti-SV40T (tsA58 temp sensitive) (Puro) Virus | LV630 | View | CMV | Puro | Inquire |
| Lenti-SV40T (tsA58 temp sensitive) Virus | LV680 | View |
CMV | Inquire | |
| Adenovirus | |||||
| Adeno-SV40T Virus | G210 | View | CMV | Puro | Inquire |
| Retrovirus | |||||
| Retro-SV40 Virus | G212 | View | 5'LTR | Neo | Inquire |
| Retro-SV40LT Virus | RVP010 | View | CMV | Puro | Inquire |
Recombinant hTERT Virus
| Product Name | Cat. No. | Vector Map | Promoter | Marker | Price |
|---|---|---|---|---|---|
| Lentivirus | |||||
| Lenti-hTERT-Puro Virus | LV615 | View | CMV | Puro | Inquire |
| Lenti-hTERT-Neo Virus | LV622 | View | CMV | Neo | Inquire |
| Lenti-hTERT Virus | LV674 | View | CMV | Inquire | |
| Lenti-hTERT-2A-CDK4 Virus | LV677 | View | CMV | Puro | Inquire |
| Lenti-EF1a-hTERT Virus | LV616 | View | EF1a | Puro | Inquire |
| Lenti-EF1a-hTERT-Hygro Virus | LV632 | View | EF1a | Hygro | Inquire |
| Adenovirus | |||||
| Adeno-hTERT Virus | G205 | View | CMV | Inquire | |
| Adeno-hTERT Antisense Virus | G208 | View | CMV | Inquire | |
Additional Immortalization Viruses
| Product Name | Cat. No. | Vector Map | Application | Price |
|---|---|---|---|---|
| HPV E6/E7 | ||||
| Lenti-HPV E6/E7 (Puro) Virus | LV617 | View | Immortalize keratinocytes | Inquire |
| Lenti-HPV E6/E7 Virus | LV667 | View | Inquire | |
| EBV genes (Epstein-Barr Virus) | ||||
| EBV Virus (Wild-Type) | G229 | N/A | Immortalize B lymphocytes, and sometimes T lymphocytes | Inquire |
| Adenoviral E1A-E1B | ||||
| E1A-E1B Lentivirus | LV652 | View | Immortalize a wide range of primary rodent cells, including amniotic fluid cells, epithelial cells, fibroblasts, and kidney cells | Inquire |
| siRNA | ||||
| Lenti-Rb siRNA Virus | LV621 | View | Co-express with hTERT for better immortalization of epithelial cells | Inquire |
| Lenti-p53 siRNA Virus | LV619 | View | Inquire | |
| Bmi1 | ||||
| Lenti-Bmi1 Virus | LV608 | View | Immortalize epithelial cells | Inquire |
| CDK4 | ||||
| Lenti-hTERT-2A-CDK4 Virus | LV677 | View | Co-express with hTERT for a more authentic cell model | Inquire |
| Lenti-CDK4 Virus | LV609 | View | Inquire | |
| Myc T58A (c-Myc), Ras V12 (H-Ras) | ||||
| Lenti-Myc T58A Virus | LV618 | View | Immortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells | Inquire |
| Lenti-Ras V12 Virus | LV620 | View | Inquire | |
| HOXB8, HOXA9, HOXA10 | ||||
| Lenti-HOXB8-RFP Virus | LV638 | View | Immortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells | Inquire |
| Lenti-HOXA9-RFP Virus | LV644 | View | Inquire | |
| Lenti-HOXA10-RFP Virus | LV650 | View | Inquire | |
| Other Related Reagents | ||||
| ViralEntry™ Transduction Enhancer (100X) | G515 | Boosts lentiviral transduction | Inquire | |
What Researchers are Saying:
| “Stable proliferation over 50 passages.” |
|
“We successfully established an immortalized bottlenose dolphin kidney cell line using SV40 large T antigen lentivirus from abm… retaining epithelial morphology.”
— Tashiro et al., 2023, In Vitro Cell. Dev. Biol. - Animal. DOI: 10.1007/s11626-023-00786-y |
| “Ideal for long-term virus pathogenesis studies.” |
|
“Immortalization of feline respiratory epithelial cells using abm's SV40 lentivirus allowed differentiation in ALI culture and FHV-1 infection studies.“
— Lee et al., 2023, Virus Research. DOI: 10.1016/j.virusres.2023.199063 |
| “Reliable immortalization with preserved phenotype.” |
|
“We used abm’s Lenti-hTERT-Neo Virus along with p53 siRNA virus to immortalize human kidney cells. Stable lines were selected 48 hours post-transduction using 800 μg/mL G418.“
— Harafuji et al., 2023, Frontiers in Cell and Developmental Biology. DOI: 10.3389/fcell.2023.1270980 |
| “Immortal feline epithelial cells with >20 passages.” |
|
“Feline respiratory epithelial cell (FREC) immortalization was performed by Applied Biological Materials Inc. The resulting iFRECs could be passaged over 20 times, while primary FRECs did not survive beyond two. Expression of SV40T and HPV16 E6/E7 was confirmed by RT-qPCR at passage 8.“
— Lee et al., 2023, Virus Research. DOI: 10.1016/j.virusres.2023.199064 |
Additional Information
-
Cell Immortalization Handbook
This Handbook provides an overview of methods, protocols, and reagents. -
Cell Immortalization Workflow
For more information, visit our Cell Immortalization Learning Resource. -
Custom Cell Immortalization Service
Our scientists can immortalize your cell line of choice.
Top Publications
Establishment and characterization of imortalized sweat gland myoepithelial cells.
Hayakawa T. et al.
Scientific Reports (2022)
doi: 10.1038/s41598-021-03991-5
Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency.
Cacchiarelli D. et al.
Cell (2015)
doi: 10.1016/j.cell.2015.06.016
Tumor-penetrating therapy for β5 integrin-rich pancreas cancer.
Hurtado T. et al.
Nature Communications (2021)
doi: 10.1038/s41467-021-21858-1