Adeno-SV40T Virus
| Cat. No. | G210 |
| Name | Adeno-SV40T Virus |
| Unit | 1 ml |
| Unpacking and Storage Instructions |
For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Description |
Standard Titer (106 pfu/ml) Recombinant Adenovirus expressing the SV40 large and small T antigens. |
| Application |
Cell immortalization. |
| Expression System Type | Adenovirus |
| Note |
Not for resale without prior written consent of abm. |
| Caution |
This product is distributed for laboratory |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G210 |
Print/Download Datasheet
| What are the units used to measure lentivirus, retrovirus, AAV and adenovirus particles? | |
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Lentivirus and retrovirus titer can be measured as CFU/ml (Colony Forming Units per ml) AAV titer is measured as GC/ml (Genomic Copies per ml). Adenovirus titer is measured as PFU/ml (Plaque Forming Units per ml). The concentration for all types of viruses can also be classified as IU/ml (Infectious Units per ml). Ultimately, the units refer to the viral particles and different units reflect the different assays involved.
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What PCR primers can I use for SV40 detection?
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PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
Expected band size: 792bp
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| What qPCR primers can I use for SV40 detection? | |
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SV40 Forward Primer Sequence
TTCCCTGACCTGAAGGCAAATC
SV40 Reverse Primer Sequence
GGCTGAACTTTGAGCTAGGAGTAG
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- Wu, L et al. " Immortalized Mouse Floxed Bmp2 Dental Papilla Mesenchymal Cell Lines Preserve Odontoblastic Phenotype and Respond to BMP2. J. Cell" Physiol 225:132-139 (2010). DOI: 10.1002/jcp.22204. Application: Immortalization.
- Olyslaegers, D et al. "Generation and characterization of feline arterial and genous endothelial cell lines for the study of the vascular endothelium" BMC Veterinary Research 9:170 (2013). DOI: 10.1186/1746-6148-9-170. Application: Immortalization.
- Desmarets, L et al. "Establishment of feline intestinal epithelial cell cultures for the propagation and study of feline" Veterinary Research 44:71 (2013). DOI: 10.1186/1297-9716-44-71. Application: Immortalization.
- Zhao, Y et al. "microRNA response elements-regulated TRAIL expression shows specific survival-suppressing activity on bladder cancer" J. Exp. Clin. Cancer Res. 32 (10): (2013). DOI: 10.1186/1756-9966-32-10. Application: Gene Expression.
- Hoffman, B et al. "Development and Characterization of a Human Articular Cartilage-Derived Chondrocyte Cell Line That Retains Chondrocyte Phenotype" J. Cell Physiol. 222 (2010):695-702 (0). DOI: 10.1002/jcp.21994. Application: Gene Expression.
- Balducci, L et al. "Immortalization of human adipose-derived stromal cells: production of cell lines with high growth rate, mesenchymal marker expression and capability to secrete high levels of angiogenic factors" Stem Cell Research & Therapy 3:63 (2014). DOI: 10.1186/scrt452. PubMed: 24887516. Application: Immortalization.
- Wong, SY et al. "Primary cilia can both mediate and suppress Hedgehog pathway–dependent tumorigenesis" Nat. Med. 9:1055 - 1061 (2009). DOI: doi:10.1038/nm.2011. PubMed: 19701205. Application: Immortalization.
- Wang, ZY et al. "Immortalized porcine liver sinusoidal endothelial cells: an in vitro model of xenotransplantation-induced thrombocytopenia" Xenotransplantation 19:249-55 (2012). PubMed: 22909138.
- Chen, Q et al. "Pharmacological inhibition of S-nitrosoglutathione reductase improves endothelial vasodilatory function in rats in vivo" J. Appl. Physiol. 6:752-60 (2013). DOI: 10.1152/japplphysiol.01302.2012. PubMed: 23349456. Application: Immortalization.
- Zhao, Y et al. "microRNA response elements-regulated TRAIL expression shows specific survival-suppressing activity on bladder cancer." J Exp Clin Cancer Res 32(1):10 (2013). DOI: 10.1186/1756-9966-32-10. PubMed: 23442927. Application: Immortalization.
- Liu, J et al. "Oleanolic acid inhibits proliferation and invasiveness of Kras-transformed cells via autophagy" J Nutr Biochem 25(11):1154-1160 (2014). DOI: 10.1016/j.jnutbio.2014.06.006. PubMed: 25172632.
- Nauwynck, H.J. et al. "Generation and Characterization of feline arterial and cenous endotheilial cell lines for the study of the vascular endothelium " Biomed Central 9: (2013). DOI: 10.1186/1746-6148-9-170. Application: Gene Delivery.
- Laval, et al. "Equine Herpesvirus Type 1 Enhances Viral Replication in CD172a+ Monocytic Cells upon Adhesion to Endothelial Cells" Journal of Virology 21:10912-10923 (2015). DOI: 10.1128/JVI.01589-15.
- Liu, et al. "Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal and Osteoblast Cell Lines Retain Their Primary Characteristics" Journal of Cellular Physiology 11:2581-2587 (2015). DOI: 10.1002/jcp.25008.
- Coleman, S et al. "A Homolog Pentameric Complex Dictates Viral Epithelial Tropism, Pathogenicity and Congenital Infection Rate in Guinea Pig Cytomegalovirus" PLoS Pathog 12.7:e1005755 (2016). DOI: 10.1371/journal.ppat.1005755. Application: Immortalization.
- Jester, JV et al. "PPARγ Regulates Mouse Meibocyte Differentiation and Lipid Synthesis" The Ocular Surface 14.4:484-494 (2016). DOI: 10.1016/j.jtos.2016.08.001. Application: Immortalization.
- Wang, ZY et al. "Immunogenicity of Renal Microvascular Endothelial Cells From Genetically Modified Pigs" Transplantation 100.3:533-537 (2016). DOI: 10.1097/TP.0000000000001070. Application: Immortalization.
- Cao, M., Yuan, C., Chen, X., He, G., Chen, T., Zong, J., Shen, C., Wang, N., Zhao, Y., Zhang, B., Li, C., & Zhou, X. (2024). METTL3 deficiency leads to ovarian insufficiency due to IL-1β overexpression in theca cells. Free Radical Biology and Medicine, 222, 72–84. https://doi.org/10.1016/j.freeradbiomed.2024.05.048
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