Custom CRISPR Multiplex sgRNA Vector | Applied Biological Materials Inc

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Services

Custom CRISPR Multiplex sgRNA Vectors

abm’s Custom CRISPR Multiplex sgRNA system allows for optimal expression of multiple sgRNAs from alternating U6 and H1 RNA pol III promoters on a single lentiviral vector. This allows for expression of multiple sgRNAs targeting a single gene or multiple sgRNAs targeting multiple different genes. sgRNAs may be designed for use with any Cas9 variant, which is expressed from a separate vector. Together, the Multiplex sgRNA Vectors expand the CRISPR toolkit allowing for highly diverse gene editing applications. Inquire with us below to get started on your custom CRISPR project.

Applications:

Gene Activation using dCas9-VPR
Gene Repression using dCas9-KRAB
Epigenetic Gene Activation using dCas9-TET1CD
Epigenetic Gene Repression using dCas9-DNMT3A
Gene Knockout using spCas9

Service Details

Cloning Services

sgRNA Service (for spCas9)	No. of sgRNAs	Unit	Cat. No.	Price
Custom CRISPR Dual sgRNA Lentivector (for spCas9)	2	1.0 µg	C420
Custom CRISPR Triple sgRNA Lentivector (for spCas9)	3	1.0 µg	C421
Custom CRISPR Quad sgRNA Lentivector (for spCas9)	4	1.0 µg	C422
Custom CRISPR Multiplex sgRNA Lentivector (for spCas9)	>4	1.0 µg	C423	Starting at

sgRNA Service (for saCas9)	No. of sgRNAs	Unit	Cat. No.	Price
Custom CRISPR Dual sgRNA Lentivector (for saCas9)	2	1.0 µg	C511
Custom CRISPR Triple sgRNA Lentivector (for saCas9)	3	1.0 µg	C512
Custom CRISPR Quad sgRNA Lentivector (for saCas9)	4	1.0 µg	C513
Custom CRISPR Multiplex sgRNA Lentivector (for saCas9)	>4	1.0 µg	C514	Starting at

Lentivirus Packaging Services

Scale	Application	Purification	Typical Titer	Volume	Cat. No.
Mini	Cell culture	Supernatant	>2x10⁸ IU/ml	3 x 250 μl	LV001‑b
Regular	Cell culture	Gradient	>2x10⁹ IU/ml	4 x 100 μl	LV001‑c
Ultra-Pure	In vivo	Ultracentrifuge	>5x10¹⁰ IU/ml	10 x 50 μl	LV001‑d
96-Well	Cell culture	Supernatant	>1x10⁷ IU/ml	100 μl/well	LV001‑e

Additional Info

* The vector for our CRISPR Multiplex sgRNA lentivectors is pLenti-Multi-sgRNA-PGK-Neo.

Additional Resources

Documents

FAQs

What promoter(s) are used in the Multiplex sgRNA vectors?

sgRNAs are expressed from alternating U6 and H1 promoters.

What is the approximate time frame to generate CRISPR lentiviral constructs?

The typical lead time is around 2-3 weeks for CRISPR lentivectors and 2-3 weeks for lentiviruses. Please inquire for a more accurate lead time.

Citations

01	Metzger D. et al. "Host Cell Transcriptional Tuning with CRISPR/dCas9 to Mitigate the Effects of Toxin Exposure." ACS Synth. Biol. (2022) 11, 3657-3668. doi: 10.1021/acssynbio.2c00214
02	Shirasaki, T. et al. "Nonlytic Quasi-Enveloped Hepatovirus Release Is Facilitated by pX Protein Interaction with the E3 Ubiquitin Ligase ITCH." J. Virol. (2022) 96(21). doi: 10.1128/jvi.01195-22