Custom CRISPR sgRNA AAV Vectors & Viruses
abm provides custom AAV vectors and viruses for CRISPR/Cas9 experiments, offering both All-in-One (Cas9 and sgRNA expressing) and sgRNA-only expression systems. Our extensive selection of AAV serotypes includes 1-11, AAV2/8, AAV2/9n, AAV2.7m8, PHP.eB, PHP.S, shH10, and Anc80L65. Choose from our All-in-One system (featuring the saCas9 variant) or our sgRNA-only expression system and pair the latter with a Cas9-Expressing Stable Cell Line or spCas9 Nuclease or saCas9 Nuclease. Inquire with us below to get started on your custom CRISPR project.
Advantages of the AAV System:
- Used as a promising candidate for gene therapy
- Does not elicit significant immune responses in vivo
- Broad tropism - tissue specificity with different AAV serotypes
- No integration into the host genome
- Ability to transduce both proliferating and quiescent cells
- Long-term expression in non-dividing cells
Service Details
Cloning Services
| Service | Vector | Unit | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR sgRNA AAV vector (for spCas9) – single target | pAAV-U6-sgRNAsp-GFP | 1.0 µg | C303 | |
| Custom CRISPR sgRNA AAV vector (for spCas9) – set of three targets | pAAV-U6-sgRNAsp-GFP | 3 x 1.0 µg | C302 | |
| Custom CRISPR sgRNA AAV vector (for saCas9) – single target | pAAV-U6-sgRNAsa-GFP | 1.0 µg | C305 | |
| Custom CRISPR sgRNA AAV vector (for saCas9) – set of three targets | pAAV-U6-sgRNAsa-GFP | 3 x 1.0 µg | C304 | |
| Custom CRISPR Cas9 All-in-One AAV vector (for saCas9) – single target | pAAV-PGK-saCas9-U6-sgRNAsa | 1.0 µg | C307 | |
| Custom CRISPR Cas9 All-in-One AAV vector (for saCas9) – set of three targets | pAAV-PGK-saCas9-U6-sgRNAsa | 3 x 1.0 µg | C306 | |
| Custom CRISPR sgRNA AAV vector (for AaCas12b) – set of three targets | pAAV-PGK-GFP-U6-sgRNAaa | 3 x 1.0 µg | G6103 |
AAV Packaging Services
- AAV serotypes 3, 4, and 6 typically produce lower yields compared to other serotypes. As such, we can only guarantee 50% of the minimum titers listed in the table above.
Additional Info
Available Custom CRISPR Vectors & Viruses
| AAV Vector/Virus | Lentivector/Virus | Non-Viral Vector | Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| All-in-One with AaCas12b | - | Available | - | - |
| sgRNA for AaCas12b | Available | Available | - | - |
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AAV Serotype Selection Chart
Additional Resources
Documents
FAQs
| Are your CRISPR sgRNA AAV replication deficient? |
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Yes, the replication and capsid genes are provided in trans when the AAV are produced, therefore the packaged virion only has the ITR sequences and the sgRNA (and All-in-One AAV have saCas9). Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
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| What is the packaging capacity for AAV? |
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The maximum insert size is < 4.4 kb.
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| Is AAV stable? What is the recommended storage temperature? |
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It is recommended to store AAV at -80˚C for long term storage. For short term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
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Citations
| 01 | Fleck JS, et al. "Inferring and perturbing cell fate regulomes in human brain organoids." Nature (2022). doi: 10.1038/s41586-022-05279-8 |
| 02 | Das A, et al. "Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus." Nature Microbiology (2020). doi: 10.1038/s41564-020-0727-8 |
| 03 | Yamane D, et al. "Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses." Nature Microbiology (2019). doi: 10.1038/s41564-019-0425-6 |