pAAV-PGK-saCas9-U6-sgRNAsa

                            LOCUS       dna                      8020 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    823..828
                     /dbxref="REBASE:NdeI"
     ORF             1176..4673
                     /sequence="ORF_2 rf(3)"
     ORF             1341..4826
                     /sequence="ORF_3 rf(6)"
     misc_binding    1398..1403
                     /dbxref="REBASE:EcoRI"
     misc_binding    1542..1547
                     /dbxref="REBASE:ClaI"
     misc_binding    1914..1919
                     /dbxref="REBASE:PstI"
     misc_binding    2813..2818
                     /dbxref="REBASE:HindIII"
     misc_binding    4674..4679
                     /dbxref="REBASE:XhoI"
     Rep_Origin      5332..5951
                     /gene="pBR322 origin"
     Promoter        6604..6653
                     /gene="NEOKAN prom"
     ORF             6742..7536
                     /sequence="ORF_1 rf(1)"
     Marker          6745..7533
                     /gene="NTP_II marker"
     Rep_Origin      7641..7946
                     /gene="f1 origin"
BASE COUNT    2224 a   2055 c   2158 g   1583 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc     
      481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg     
      541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta     
      601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggaa     
      661 aaaaatctcg ccaacaagtt gacgagataa acacggcatt ttgccttgtt ttagtagatt     
      721 ctgtttccag agtactaaaa cgggtcttcc catgggaaga cggcgtcctt tccacaagat     
      781 atataaagcc aagaaatcga aatactttca agttacggta agcatatgat agtccatttt     
      841 aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt     
      901 ttgtactaat atctttgtgt ttacagtcaa attaattcca attatctctc taacagcctt     
      961 gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcaccgg taggcgccaa     
     1021 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag     
     1081 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc     
     1141 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc     
     1201 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg     
     1261 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga     
     1321 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca     
     1381 tctccgggcc tttcgacgaa ttcgccacca tggccccaaa gaagaagcgg aaggtcggta     
     1441 tccacggagt cccagcagcc aagcggaact acatcctggg cctggacatc ggcatcacca     
     1501 gcgtgggcta cggcatcatc gactacgaga cacgggacgt gatcgatgcc ggcgtgcggc     
     1561 tgttcaaaga ggccaacgtg gaaaacaacg agggcaggcg gagcaagaga ggcgccagaa     
     1621 ggctgaagcg gcggaggcgg catagaatcc agagagtgaa gaagctgctg ttcgactaca     
     1681 acctgctgac cgaccacagc gagctgagcg gcatcaaccc ctacgaggcc agagtgaagg     
     1741 gcctgagcca gaagctgagc gaggaagagt tctctgccgc cctgctgcac ctggccaaga     
     1801 gaagaggcgt gcacaacgtg aacgaggtgg aagaggacac cggcaacgag ctgtccacca     
     1861 aagagcagat cagccggaac agcaaggccc tggaagagaa atacgtggcc gaactgcagc     
     1921 tggaacggct gaagaaagac ggcgaagtgc ggggcagcat caacagattc aagaccagcg     
     1981 actacgtgaa agaagccaaa cagctgctga aggtgcagaa ggcctaccac cagctggacc     
     2041 agagcttcat cgacacctac atcgacctgc tggaaacccg gcggacctac tatgagggac     
     2101 ctggcgaggg cagccccttc ggctggaagg acatcaaaga atggtacgag atgctgatgg     
     2161 gccactgcac ctacttcccc gaggaactgc ggagcgtgaa gtacgcctac aacgccgacc     
     2221 tgtacaacgc cctgaacgac ctgaacaatc tcgtgatcac cagggacgag aacgagaagc     
     2281 tggaatatta cgagaagttc cagatcatcg agaacgtgtt caagcagaag aagaagccca     
     2341 ccctgaagca gatcgccaaa gaaatcctcg tgaacgaaga ggatattaag ggctacagag     
     2401 tgaccagcac cggcaagccc gagttcacca acctgaaggt gtaccacgac atcaaggaca     
     2461 ttaccgcccg gaaagagatt attgagaacg ccgagctgct ggatcagatt gccaagatcc     
     2521 tgaccatcta ccagagcagc gaggacatcc aggaagaact gaccaatctg aactccgagc     
     2581 tgacccagga agagatcgag cagatctcta atctgaaggg ctataccggc acccacaacc     
     2641 tgagcctgaa ggccatcaac ctgatcctgg acgagctgtg gcacaccaac gacaaccaga     
     2701 tcgctatctt caaccggctg aagctggtgc ccaagaaggt ggacctgtcc cagcagaaag     
     2761 agatccccac caccctggtg gacgacttca tcctgagccc cgtcgtgaag agaagcttca     
     2821 tccagagcat caaagtgatc aacgccatca tcaagaagta cggcctgccc aacgacatca     
     2881 ttatcgagct ggcccgcgag aagaactcca aggacgccca gaaaatgatc aacgagatgc     
     2941 agaagcggaa ccggcagacc aacgagcgga tcgaggaaat catccggacc accggcaaag     
     3001 agaacgccaa gtacctgatc gagaagatca agctgcacga catgcaggaa ggcaagtgcc     
     3061 tgtacagcct ggaagccatc cctctggaag atctgctgaa caaccccttc aactatgagg     
     3121 tggaccacat catccccaga agcgtgtcct tcgacaacag cttcaacaac aaggtgctcg     
     3181 tgaagcagga agaaaacagc aagaagggca accggacccc attccagtac ctgagcagca     
     3241 gcgacagcaa gatcagctac gaaaccttca agaagcacat cctgaatctg gccaagggca     
     3301 agggcagaat cagcaagacc aagaaagagt atctgctgga agaacgggac atcaacaggt     
     3361 tctccgtgca gaaagacttc atcaaccgga acctggtgga taccagatac gccaccagag     
     3421 gcctgatgaa cctgctgcgg agctacttca gagtgaacaa cctggacgtg aaagtgaagt     
     3481 ccatcaatgg cggcttcacc agctttctgc ggcggaagtg gaagtttaag aaagagcgga     
     3541 acaaggggta caagcaccac gccgaggacg ccctgatcat tgccaacgcc gatttcatct     
     3601 tcaaagagtg gaagaaactg gacaaggcca aaaaagtgat ggaaaaccag atgttcgagg     
     3661 aaaagcaggc cgagagcatg cccgagatcg aaaccgagca ggagtacaaa gagatcttca     
     3721 tcacccccca ccagatcaag cacattaagg acttcaagga ctacaagtac agccaccggg     
     3781 tggacaagaa gcctaataga gagctgatta acgacaccct gtactccacc cggaaggacg     
     3841 acaagggcaa caccctgatc gtgaacaatc tgaacggcct gtacgacaag gacaatgaca     
     3901 agctgaaaaa gctgatcaac aagagccccg aaaagctgct gatgtaccac cacgaccccc     
     3961 agacctacca gaaactgaag ctgattatgg aacagtacgg cgacgagaag aatcccctgt     
     4021 acaagtacta cgaggaaacc gggaactacc tgaccaagta ctccaaaaag gacaacggcc     
     4081 ccgtgatcaa gaagattaag tattacggca acaaactgaa cgcccatctg gacatcaccg     
     4141 acgactaccc caacagcaga aacaaggtcg tgaagctgtc cctgaagccc tacagattcg     
     4201 acgtgtacct ggacaatggc gtgtacaagt tcgtgaccgt gaagaatctg gatgtgatca     
     4261 aaaaagaaaa ctactacgaa gtgaatagca agtgctatga ggaagctaag aagctgaaga     
     4321 agatcagcaa ccaggccgag tttatcgcct ccttctacaa caacgatctg atcaagatca     
     4381 acggcgagct gtatagagtg atcggcgtga acaacgacct gctgaaccgg atcgaagtga     
     4441 acatgatcga catcacctac cgcgagtacc tggaaaacat gaacgacaag aggcccccca     
     4501 ggatcattaa gacaatcgcc tccaagaccc agagcattaa gaagtacagc acagacattc     
     4561 tgggcaacct gtatgaagtg aaatctaaga agcaccctca gatcatcaaa aagggcaaaa     
     4621 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc taactcgaga     
     4681 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa     
     4741 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat     
     4801 aaacaagtta ataacaacaa ttccattcat tttatgtttc aggttcaggg ggagatgtgg     
     4861 gaggtttttt aaagcaagta aaacctctac aaatgtggta gtcgaaattc ccgataagga     
     4921 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg     
     4981 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg     
     5041 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag     
     5101 cgcgcaggga tcccggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag     
     5161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc     
     5221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg     
     5281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct     
     5341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca     
     5401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct     
     5461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc     
     5521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt     
     5581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc     
     5641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc     
     5701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg     
     5761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc     
     5821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     
     5881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     
     5941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat     
     6001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag     
     6061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat     
     6121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc     
     6181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat     
     6241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag     
     6301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg     
     6361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgaaaaagg     
     6421 atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg     
     6481 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag     
     6541 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc     
     6601 gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac     
     6661 tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag     
     6721 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc     
     6781 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat     
     6841 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg     
     6901 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg     
     6961 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta     
     7021 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta     
     7081 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc     
     7141 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc     
     7201 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg     
     7261 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg     
     7321 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt     
     7381 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc     
     7441 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc     
     7501 atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt tttgttaaat     
     7561 cagctcattt tttaaccaat aggccgaaat cggcaacatc ccttataaat caaaagaata     
     7621 gaccgcgata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt     
     7681 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc     
     7741 atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc ggaaccctaa     
     7801 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg     
     7861 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt     
     7921 aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg ccattcagga     
     7981 tcgaattaat tcttaattaa catcatcaat aatatacctt                           
//