pAAV-U6-sgRNAsp-GFP

                            LOCUS       dna                      5886 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    457..462
                     /dbxref="REBASE:HincII"
     misc_binding    951..956
                     /dbxref="REBASE:StuI"
     Reporter        1176..1892
                     /gene="EGFP reporter"
     ORF             1176..1895
                     /sequence="ORF_1 rf(3)"
     misc_binding    2293..2298
                     /dbxref="REBASE:NdeI"
     misc_binding    2481..2486
                     /dbxref="REBASE:EcoRV"
     misc_binding    2487..2492
                     /dbxref="REBASE:XbaI"
     misc_binding    2974..2979
                     /dbxref="REBASE:BamHI"
     Rep_Origin      3198..3817
                     /gene="pBR322 origin"
     Promoter        4470..4519
                     /gene="NEOKAN prom"
     misc_binding    4577..4582
                     /dbxref="REBASE:BclI"
     ORF             4608..5402
                     /sequence="ORF_2 rf(3)"
     Marker          4611..5399
                     /gene="NTP_II marker"
     Rep_Origin      5507..5812
                     /gene="f1 origin"
BASE COUNT    1416 a   1515 c   1630 g   1325 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc     
      481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg     
      541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta     
      601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctagggg     
      661 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc     
      721 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc     
      781 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg     
      841 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt     
      901 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg     
      961 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt     
     1021 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc     
     1081 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc     
     1141 tcatctccgg gcctttcggc tagcacctcg ccaccatggt gagcaagggc gaggagctgt     
     1201 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca     
     1261 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct     
     1321 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg     
     1381 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca     
     1441 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga     
     1501 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca     
     1561 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc     
     1621 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc     
     1681 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca     
     1741 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga     
     1801 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg     
     1861 ggatcactct cggcatggac gagctgtaca agtaactcga gctgtgcctt ctagttgcca     
     1921 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac     
     1981 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat     
     2041 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca     
     2101 tgctggggac aattggaggg cctatttccc atgattcctt catatttgca tatacgatac     
     2161 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa     
     2221 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt     
     2281 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt gggtttatat     
     2341 atcttgtgga aaggacgaaa caccggagtc ttcaccggtg aagacgcgtt ttagagctag     
     2401 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg     
     2461 tgcttttttt tatcggatct gatatctcta gactcgagta cccatacgac gtcccagact     
     2521 acgctacgcg tcttaaggcg atcgcagaca tgataagata cattgatgag tttggacaaa     
     2581 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt     
     2641 tatttgtaac cattataagc tgcaataaac aagttaataa caacaattcc attcatttta     
     2701 tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat     
     2761 gtggtagtcg aaattcccga taaggatctt cctagagcat ggctacgtag ataagtagca     
     2821 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct     
     2881 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc     
     2941 ccgggcggcc tcagtgagcg agcgagcgcg cagggatccc ggtgtgaaat accgcacaga     
     3001 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg     
     3061 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta     
     3121 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc     
     3181 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     
     3241 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     
     3301 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     
     3361 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     
     3421 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     
     3481 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     
     3541 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     
     3601 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta     
     3661 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga     
     3721 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg     
     3781 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag     
     3841 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc     
     3901 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact     
     3961 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt     
     4021 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta     
     4081 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta     
     4141 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc     
     4201 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat     
     4261 agtttgcgca acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca     
     4321 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg     
     4381 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag     
     4441 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta     
     4501 aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc     
     4561 gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag     
     4621 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg     
     4681 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc     
     4741 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag     
     4801 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca     
     4861 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat     
     4921 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata     
     4981 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac     
     5041 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc     
     5101 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg     
     5161 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg     
     5221 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta     
     5281 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg     
     5341 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct     
     5401 gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc     
     5461 aacatccctt ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg     
     5521 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat     
     5581 cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc     
     5641 cgtaaagctc taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag     
     5701 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg     
     5761 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac     
     5821 agggcgcgtc cattcgccat tcaggatcga attaattctt aattaacatc atcaataata     
     5881 tacctt                                                                
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