pAAV-U6-sgRNAsa-GFP

                            LOCUS       dna                      5862 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      162..310
                     /gene="Encap other"
     Promoter        365..393
                     /gene="amp prom"
     misc_binding    457..462
                     /dbxref="REBASE:HpaI"
     misc_binding    951..956
                     /dbxref="REBASE:StuI"
     Reporter        1176..1892
                     /gene="EGFP reporter"
     ORF             1176..1895
                     /sequence="ORF_1 rf(3)"
     misc_binding    2293..2298
                     /dbxref="REBASE:NdeI"
     misc_binding    2463..2468
                     /dbxref="REBASE:XbaI"
     misc_binding    2950..2955
                     /dbxref="REBASE:BamHI"
     Rep_Origin      3174..3793
                     /gene="pBR322 origin"
     Promoter        4446..4495
                     /gene="NEOKAN prom"
     misc_binding    4553..4558
                     /dbxref="REBASE:BclI"
     ORF             4584..5378
                     /sequence="ORF_2 rf(3)"
     Marker          4587..5375
                     /gene="NTP_II marker"
     Rep_Origin      5483..5788
                     /gene="f1 origin"
BASE COUNT    1404 a   1515 c   1621 g   1322 t    0 others
ORIGIN
        1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt     
       61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa     
      121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg     
      181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt     
      241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg     
      301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag     
      361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     
      421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc     
      481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg     
      541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta     
      601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctagggg     
      661 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc     
      721 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc     
      781 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg     
      841 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt     
      901 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg     
      961 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt     
     1021 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc     
     1081 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc     
     1141 tcatctccgg gcctttcggc tagcacctcg ccaccatggt gagcaagggc gaggagctgt     
     1201 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca     
     1261 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct     
     1321 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg     
     1381 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca     
     1441 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga     
     1501 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca     
     1561 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc     
     1621 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc     
     1681 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca     
     1741 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga     
     1801 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg     
     1861 ggatcactct cggcatggac gagctgtaca agtaactcga gctgtgcctt ctagttgcca     
     1921 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac     
     1981 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat     
     2041 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca     
     2101 tgctggggac aattggaggg cctatttccc atgattcctt catatttgca tatacgatac     
     2161 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa     
     2221 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt     
     2281 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat     
     2341 atcttgtgga aaggacgccg tcttcccatg ggaagacccg ttttagtact ctggaaacag     
     2401 aatctactaa aacaaggcaa aatgccgtgt ttatctcgtc aacttgttgg cgagattttt     
     2461 tttctagact cgagtaccca tacgacgtcc cagactacgc tacgcgtctt aaggcgatcg     
     2521 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa     
     2581 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca     
     2641 ataaacaagt taataacaac aattccattc attttatgtt tcaggttcag ggggagatgt     
     2701 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tagtcgaaat tcccgataag     
     2761 gatcttccta gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa     
     2821 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc     
     2881 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg     
     2941 agcgcgcagg gatcccggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc     
     3001 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     
     3061 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     
     3121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     
     3181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     
     3241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     
     3301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     
     3361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     
     3421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     
     3481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     
     3541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     
     3601 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag     
     3661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     
     3721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     
     3781 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     
     3841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     
     3901 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     
     3961 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     
     4021 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     
     4081 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     
     4141 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     
     4201 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgaaaaa     
     4261 ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc     
     4321 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc     
     4381 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa     
     4441 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa     
     4501 actggatggc tttctcgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag     
     4561 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg     
     4621 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg     
     4681 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc     
     4741 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga     
     4801 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc     
     4861 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag     
     4921 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat     
     4981 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg     
     5041 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca     
     5101 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct     
     5161 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg     
     5221 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg     
     5281 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc     
     5341 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa     
     5401 atcagctcat tttttaacca ataggccgaa atcggcaaca tcccttataa atcaaaagaa     
     5461 tagaccgcga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac     
     5521 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa     
     5581 ccatcaccca aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct     
     5641 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa     
     5701 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc     
     5761 gtaaccacca cacccgcgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag     
     5821 gatcgaatta attcttaatt aacatcatca ataatatacc tt                        
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