Mycoplasma Detection

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All Products

abm’s Mycoplasma detection kits offer fast, sensitive, and reliable screening to protect your cell cultures. The Mycoplasma PCR and Pro PCR Detection Kits detect over 200 and 300 strains respectively, with the Pro version featuring an internal control for added accuracy. For ultra sensitivity, the Mycoplasma qPCR Detection Kit detects as few as 10 copies directly from media. All three outperform competitors in sensitivity, speed, and value.

Get results in 1 hr

Detects 200+ Mycoplasma species

Easy-to-use (no DNA isolation needed)

Product List

Product Name	Cat. No.	Size	Price
Mycoplasma PCR Detection Kit	G238	100 reactions	Inquiry
Mycoplasma Pro PCR Detection Kit	G239	100 reactions	*
Mycoplasma qPCR Detection Kit	G240	100 reactions	*
Mycoplasma PCR Detection & Elimination Bundle	G238-G398	Bundle	Inquiry
Mycoplasma Pro PCR Detection & Elimination Bundle	G239-G398	Bundle	*
Mycoplasma qPCR Detection & Elimination Bundle	G240-G398	Bundle	*

* Special Introductory Price

Unsure which kit is right for you?

	Mycoplasma PCR Detection Kit	Mycoplasma Pro PCR Detection Kit	Mycoplasma qPCR Detection Kit
Detection Method	PCR + gel	PCR + gel	Real-time qPCR
Target Coverage	200+ Mycoplasma	300+ Mycoplasma & Acholeplasma	Mycoplasma & Acholeplasma
Internal Control	No	Yes	No
Sample Prep Needed	None	None	Dilution only
Time to Results	< 2 hours	< 2 hours	< 2 hours
Electrophoresis Required	Yes	Yes	No
System Compatibility	Standard PCR	Standard PCR	qPCR (ROX-compatible)
Special Notes	Cost-effective, high performance	Broader strain coverage + internal control	Ultra-sensitive

Sensitive Detection

abm's Mycoplasma PCR Detection Kit can detect samples containing as low as 10 genome copies of Mycoplasma. abm's Mycoplasma PCR Detection Kit (Cat. No. G238) was used to detect samples containing ~10 to 100,000 genome copies of Mycoplasma. Presence of Mycoplasma was confirmed as a 450 bp band.

Confident Results

Mycoplasma Pro PCR Detection Kit (Cat. No. G239) was used to confirm the presence of contamination in five cell culture samples. Positive mycoplasma bands (300 bp – 600 bp) were detected in samples 2, 3, and 4. Internal PCR control band (180 bp) is present in all lanes which confidently determines a genuine negative result.

Reliable Amplification

Mycoplasma qPCR Detection Kit (Cat. No. G240) demonstrates a highly linear qPCR standard curve from a tenfold serial dilution of positive control DNA (10⁷ to 1 copy). qPCR amplification was performed across eight dilution points, and the resulting Ct values showed a strong linear correlation with log₁₀(copy number), indicating excellent amplification efficiency and a broad dynamic range. No amplification was observed in the No Template Control (NTC), confirming the assay’s high specificity.