Mycoplasma qPCR Detection Kit
| Cat. No. | G240 | ||||||||||||||||||||||||
| Name | Mycoplasma qPCR Detection Kit | ||||||||||||||||||||||||
| Description |
abm’s Mycoplasma qPCR Detection Kit is a powerful addition to our trusted Mycoplasma Detection line, offering rapid and ultra-sensitive detection of Mycoplasma and Acholeplasma directly from cell culture supernatant—no DNA extraction, sample preparation, or gel electrophoresis required. Capable of detecting as few as 10 copies with complete elimination of no template control (NTC) signals, this qPCR-based kit delivers results in under 2 hours. It’s the ideal solution for routine contamination screening and quality control in sensitive workflows. Branded as the #1 qPCR Mycoplasma Detection Kit, it includes our proprietary 2X MegaFi™ Pro qPCR MasterMix and a separate ROX Reference Dye for broad qPCR instrument compatibility. For details, refer to our ROX Machine Compatibility Chart. Kit Features:
Why test your cells for Mycoplasma? Due to its small size, Mycoplasma can be present in your growth media without being visibly detected. This can cause significant errors in your experiments due to its effects on:
Many publishers now request Mycoplasma testing to be reported along with manuscript submissions to ensure accuracy in data reporting. Visit our learning resources center to read more about Mycoplasma contamination. Note:
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| Unit | 100 Reactions | ||||||||||||||||||||||||
| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G240 |
| Is this kit compatible with all real-time PCR machines? | |
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The qPCR MasterMix contains dye comparable to SYBR Green™ and EvaGreen™. This makes the kit compatible with all standard SYBR-capable qPCR systems. ROX Reference Dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments. For specific instrument requirements, please refer to the Rox Machine Compatibility Chart.
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| Are there any additional sample preparations required before running the qPCR? | |
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No additional sample preparation is required. Simply dilute your cell culture supernatant sample 1:5 using the provided Nuclease-free water prior to adding it to the qPCR reaction. This helps minimize inhibitors which could affect qPCR sensitivity.
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| Can I use this kit for detecting Mycoplasma in various cell culture supernatants? | |
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Yes, this kit has been tested with common laboratory media and serums.
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| The melt peak in my sample is at a different temperature than the positive control. Are my results still valid? | |
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Yes, your results can still be accurate. Because the primers are designed to detect a broad range of mycoplasma species and strains, the resulting amplicons can have slightly different sequences and lengths, which lead to variation in melt peak temperatures.
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| What if my NTC shows amplification? | |
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Amplification in the NTC typically indicates:
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| What is the limit of detection (LoD) of this kit? | |
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This kit reliably detects as few as 10 copies of mycoplasma DNA per reaction. This is based on validation data showing consistent amplification up to Ct 35. Signals beyond Ct 35 are considered below the detection threshold and interpreted as negative.
If you suspect low-level contamination, we recommend continuing to culture the cells for 48–72 hours and re-testing, as the mycoplasma population may be below the detectable limit in early infections. |
| What are the species and strains detected using Mycoplasma qPCR Detection Kit (Cat. No. G240)? | |
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The kit covers more than 300 species and strains of Mycoplasma including the most common species M. arginini, M. pneumoniae, M. hyorhinis, M. orale, and Acholeplasma laidlawii. Please refer to our Mycoplasma Strains Guide in order to determine which detection kit satisfies your experimental needs. If you cannot find the particular species or strain on the list, you can also email us at technical@abmgood.comand we can manually verify if the kit would detect your particular strain.
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| Can samples be collected in advance and stored? | |
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Collected samples can be stored at 4°C for 3-5 days before testing, or -20°C if planning to store for a longer period of time. Please note, samples should be drawn from cells that have been in culture for at least 48-72 hours un-disturbed and are at least 80% confluent.
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| Our cells are grown in suspension and would not achieve 80% confluence, is there a target cell density we should achieve to ensure Mycoplasma is detected? | |
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Mycoplasma testing is more dependent on how long the cells have been in culture. Seeding density does not play a major role when using the detection kit. We recommend cells be kept in culture for 48-72 hours without changing the media so as to detect Mycoplasma in culture supernatant.
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| My culture media contains antibiotics, will that be an issue? | |
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It is fine to test supernatant containing antibiotics. Please ensure that the culture has been in incubation for at least 48-72 hours prior to sample collection.
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