BlasTaq™ 2X PCR MasterMix
| Cat. No. | G895 | ||||||
| Name | BlasTaq™ 2X PCR MasterMix | ||||||
| Unit | 800 rxn (10.0 ml) | ||||||
| Category | PCR Polymerase | ||||||
| Description |
Blast through a routine 2 hour PCR run in only 20 minutes! BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s BlasTaq™ DNA Polymerase in a uniquely-formulated buffer with gel loading dye. BlasTaq™ DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors. Product Features:
1 MasterMix contains 1.5 mM Mg2+
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| Application |
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| Concentration | 2X | ||||||
| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G895 |
| Does the dye in the MasterMix affect downstream applications? | |
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The loading dye does not interfere with downstream cloning experiments.
However, PCR clean-up is still advised prior to cloning due to the presence of excess primers, salts, and other buffer components that could potentially affect cloning efficiency.
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| I am observing positive results in my negative controls (no template added) when attempting to detect gene sequences in a bacterial strain. I suspect this may be due to residual bacterial DNA from the DNA polymerase. What steps should I take to address this issue? | |
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The presence of residual bacterial DNA is quite common in commercially available DNA polymerases, as most are expressed and purified using E. coli recombinant systems. In this case, we recommend switching to our Ultra-Pure BlasTaq™ 2X PCR MasterMix (Cat. No. G885). This product undergoes a rigorous multi-step purification protocol utilizing physical, chemical, and enzymatic methods to maximize the removal of contaminating genomic DNA.
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| How does BlasTaq™ differ from regular Taq polymerase? | |
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BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
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| How should I store this product? | |
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This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
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| Can I use BlasTaq™ for TA cloning? | |
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Yes, BlasTaq™ DNA Polymerase produces 3’-dA-tailed amplicons, which makes it compatible with TA cloning vectors.
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| Can I use BlasTaq™ for high-GC templates? | |
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Yes, BlasTaq™ is designed to handle a variety of templates, including those with high GC content. For high-GC templates, it is recommended to increase the initial denaturation step to 5 minutes to ensure complete denaturation of the template.
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| How do I know if my PCR reaction requires modification of the buffer or thermocycling conditions? | |
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BlasTaq™'s specialized buffer is optimized for most PCR applications, including primer annealing at 60°C. However, if your primers do not efficiently anneal or if you are working with particularly challenging templates, you may need to optimize the annealing temperature or adjust cycling conditions based on your results.
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| Can I store my PCR reaction mix once it has been assembled? | |
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It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
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| What is the shelf life of BlasTaq™? | |
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The shelf life of BlasTaq™ is typically indicated on the product label or datasheet, but as a general rule, it is stable for up to 1-2 years when stored properly at -20°C. Be sure to check the expiration date and make sure the enzyme remains frozen when not in use.
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| What is the advantage of using BlasTaq™ 2X PCR MasterMix over individual PCR components? | |
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BlasTaq™ 2X PCR MasterMix is a ready-to-use solution that simplifies the PCR setup process. It contains both the BlasTaq™ DNA Polymerase and a uniquely formulated buffer with gel loading dye, which saves time and reduces the risk of errors during reaction preparation. This eliminates the need to add individual components like dNTPs and buffer separately, allowing for a more efficient and streamlined workflow.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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