Kodaq 2X PCR MasterMix
| Cat. No. | G883 |
| Name | Kodaq 2X PCR MasterMix |
| Unit | 800 reactions (10.0 ml) |
| Category | PCR Polymerase |
| Description |
Kodaq 2X PCR MasterMix is a ready-to-use mixture containing Kodaq DNA Polymerase and all the necessary reagents for amplification of DNA in a uniquely formulated buffer with gel loading dye. Kodaq DNA Polymerase is a next-generation enzyme engineered for superior accuracy, robustness, and high-yield amplification, making it ideal for a wide range of PCR applications. This novel polymerase features strategically designed mutations that enhance processivity, DNA binding affinity, and stability, ensuring reliable and efficient performance. With exceptional 3’-5’ exonuclease activity, Kodaq offers unmatched fidelity compared to standard polymerases. Its advanced buffer system is optimized to tolerate high A/T and G/C content while overcoming common PCR inhibitors, including those present in plant-derived samples. Product Features:
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| Note |
Suitable for long range PCR up to 18 kb from less difficult targets or up to 15 kb from genomic DNA. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G883 |
| I am observing positive results in my negative controls (no template added) when attempting to detect gene sequences in a bacterial strain. I suspect this may be due to residual bacterial DNA from the DNA polymerase. What steps should I take to address this issue? | |
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The presence of residual bacterial DNA is quite common in commercially available DNA polymerases, as most are expressed and purified using E. coli recombinant systems. In this case, we recommend switching to our Ultra-Pure BlasTaq™ 2X PCR MasterMix (Cat. No. G885). This product undergoes a rigorous multi-step purification protocol utilizing physical, chemical, and enzymatic methods to maximize the removal of contaminating genomic DNA.
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| How should I store this product? | |
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This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
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| Can I store my PCR reaction mix once it has been assembled? | |
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It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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