MegaFi™ Pro One-Step RT-PCR
| Cat. No. | G597 | ||||||||
| Name | MegaFi™ Pro One-Step RT-PCR | ||||||||
| Unit | 100 rxn | ||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||
| Description |
MegaFi™ Pro One-Step RT-PCR is a highly efficient, all-in-one solution for performing both reverse transcription and PCR amplification in a single reaction. The kit includes two essential components: the RT-PCR enzyme mix, which combines OneScript® Hot Reverse Transcriptase (Cat. No. G593), MegaFi™ Pro Fidelity DNA Polymerase (Cat. No. G886) , and RNaseOFF Ribonuclease Inhibitor (Cat. No. G138), and the 2X One-Step RT-PCR Buffer, which contains gel loading dye and all other necessary reagents. The enzyme mix ensures exceptional sensitivity and high fidelity with over 2000X lower error rates, while the buffer is optimized with stabilizers and enhancers provides flexiblity in choosing desired primers to support robust reactions across a variety of RNA templates. This streamlined system simplifies the process, offering a reliable, one-step alternative to traditional two-step RT-PCR protocols. Product Features:
|
||||||||
| Storage Condition |
Store at -20°C. |
||||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G597 |
| Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis? | |
|
Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
|
| How much RNA template should I use for cDNA synthesis? | |
|
We recommend using 1 ng to 2 μg of RNA per reaction.
|
| How should I store this product? | |
|
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
|
| Can I store my PCR reaction mix once it has been assembled? | |
|
It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
|
| What discontinued abm product is Cat. No. G597 equivalent to? | |
|
This product is abm’s next generation of One-Step RT-PCR Kits and is functionally equivalent to Cat. No. G174 and G174-dye (One-Step RT-PCR Kits), with improved performance. Contact our customer service team technical@abmgood.com for more information.
|
| What is the source of this enzyme? | |
|
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
|
-
Dik, I., Bulut, O., Avci, O., Hasoksuz, M., Palanci, H. S., Aslim, H. P., & Bulut, Z. (2023). Molecular Detection and Characterization of Bovine Noroviruses from Cattle in Konya, Turkey. Pakistan Veterinary Journal, 43(1). https://doi.org/10.29261/pakvetj/2022.089
Oz, M.E., Avci, O. & Dogan, M. Factors influencing the prevalence of acute bee paralysis virus in Apis mellifera and insights into its phylogenetic relationships. Virus Genes 61, 220–229 (2025). https://doi.org/10.1007/s11262-025-02135-5
Palancı, H. S. (2024). Resveratrolün In Vitro ortamda bovine coronavirus üzerine antiviral aktivitesinin belirlenmesi ve immünolojik gen ekspresyon seviyelerinin değerlendirilmesi. https://hdl.handle.net/20.500.12395/53198
Palancı, H. S., Avcı, O., Dik, I., Aslım, H. P., Gülbahçe, R., & Bulut, O. (2024). Serological Investigation of Bovine Enterovirus in Calves in Konya Province. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 21(1), 43-49. https://doi.org/10.32707/ercivet.1455296
Şevik M, Zerek A, Erdem İ, Yaman M. Evidence of circulating recombinants between deformed wing virus and Varroa destructor virus-1 in honey bee colonies in Türkiye. Bulletin of Entomological Research. 2024;114(5):631-641. https://doi.org/10.1017/S000748532400052X
Vasco-Julio, D., Aguilar, D., Maldonado, A., De la Torre, E., Cisneros-Montufar, M. S., Bastidas-Caldes, C., ... & De Waard, J. H. (2023). Molecular tracking of the origin of vesicular stomatitis outbreaks in 2004 and 2018, Ecuador. Veterinary Sciences, 10(3), 181. https://doi.org/10.3390/vetsci10030181