RNaseOFF Ribonuclease Inhibitor
| Cat. No. | G138 | |
| Name | RNaseOFF Ribonuclease Inhibitor | |
| Unit | 4,000 U (100 µl) | |
| Category | Molecular Biology Enzymes and Kits | |
| Description |
Same performance as competing brands, better pricing! RNaseOFF Ribonuclease Inhibitor is an advanced enzyme designed to provide superior protection for RNA in sensitive experiments. Specifically engineered to inhibit common ribonucleases (RNases), including RNase A, B, and C, it offers high-affinity protection against potential RNase contamination. This makes RNaseOFF an invaluable additive in PCR and RT-PCR applications, ensuring that RNA remains intact without inhibiting polymerase activity. Unlike traditional human RNase inhibitors, RNaseOFF is more resistant to oxidation, offering enhanced stability even under challenging conditions. It is particularly stable at low concentrations of DTT (< 1 mM), making it the optimal choice for experiments requiring minimal reducing agents. With its ability to safeguard RNA while maintaining polymerase efficiency, RNaseOFF is the ideal solution for achieving reliable, high-quality results in your research.
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| Application |
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| Concentration | 40 U/µl | |
| Storage Condition |
Store at -20°C. |
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| Note |
One unit is defined as the amount of RNaseOFF Ribonuclease Inhibitor that is required to inhibit the activity of 5 ng of RNase A by 50%.
This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G138 |
| Are all components in this product RNase-free? | |
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Yes, all components in the RNaseOFF Ribonuclease Inhibitor is RNase-free.
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| What is the shelf life of this reagent and how should it be stored? | |
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The shelf life of RNaseOFF is 1 year upon shipment of the reagent and it should be stored at -20°C for long-term storage.
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| How many reactions can be performed? | |
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The appropriate number of units per reaction will vary depending on the amount of RNase contamination that is present in the reaction mixture for any given experiment. One unit of RNaseOFF Ribonuclease Inhibitor will inhibit the activity of 5 ng of RNase by 50%; thus, the more RNase contamination present in the specific reaction, the larger number of units of RNaseOFF that will be required for the inhibition. As a general guideline, a typical amount of RNaseOFF used would be around 25-50 U per 50 µl reaction; note that this is a general guideline only and the number of units required will vary from case to case.
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| Is DTT required for the RNaseOFF Ribonuclease Inhibitor to function? | |
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No. DTT (dithiothreitol) is used to stabilize enzymes and proteins, and it is not required for the RNaseOFF Ribonuclease Inhibitor to function/inhibit ribonucleases.
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| Does RNaseOff (Cat. No. G138) inhibit RNase R activity? | |
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No, RNAseOff does not inhibit RNase R activity.
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| Why should I choose RNaseOFF over other ribonuclease inhibitors? | |
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RNaseOFF offers enhanced resistance to oxidation, making it more stable than traditional human RNase inhibitors. It is also stable at low concentrations of DTT (< 1 mM), ensuring reliable RNA protection in a variety of experimental conditions.
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| Is RNaseOFF suitable for PCR, RT-PCR, RNA isolation, and other RNA-based applications? | |
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Yes, RNaseOFF is ideal for PCR, RT-PCR, RNA isolation, cDNA synthesis, qPCR, and other RNA-based applications. It effectively prevents RNA degradation without inhibiting polymerase activity, ensuring RNA integrity and high-quality results in all these experiments.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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Carella, E., Oberto, F., Romano, A. et al. Molecular and serological investigation of Hepatitis E virus in pigs slaughtered in Northwestern Italy. BMC Vet Res 19, 21 (2023). https://doi.org/10.1186/s12917-023-03578-4
Sharma, S., & Gümüş, M. (2023). Biological and Molecular Detection of Cucumber mosaic virus (CMV) Isolates Obtained from Izmir. Akademik Ziraat Dergisi, 12(2), 199-210. http://dx.doi.org/10.29278/azd.1318370