BlasTaq™ HotStart 2X PCR MasterMix
| Cat. No. | G598 | ||
| Name | BlasTaq™ HotStart 2X PCR MasterMix | ||
| Unit | 800 rxn (10.0 ml) | ||
| Category | PCR Polymerase | ||
| Description |
BlasTaq™ HotStart 2X PCR MasterMix is a convenient, ready-to-use solution containing abm’s BlasTaq™ HotStart DNA Polymerase in a specially formulated buffer with gel loading dye. This advanced Taq polymerase is engineered for rapid extension rates, robust performance, and includes a proprietary antibody that prevents the polymerase from working at low temperatures. The HotStart feature allows for easy reaction set-up at room temperature, preventing non-specific amplification and primer dimer formation. BlasTaq™ HotStart offers enhanced processivity, higher yields, increased sensitivity, and significantly reduced reaction times—up to 70% faster than traditional wild-type Taq polymerase. During the initial denaturation, the antibody releases from the polymerase, restoring enzyme activity and reducing non-specific amplification, which ensures higher specificity and improved PCR product yields. BlasTaq™ also features 5’-3’ polymerase and exonuclease activities, but lacks 3’-5’ exonuclease activity, producing 3’-dA-tailed amplicons that are compatible with TA cloning vectors. Product Features:
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G598 |
| How does BlasTaq™ differ from regular Taq polymerase? | |
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BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
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| How should I store this product? | |
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This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
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| Can I use BlasTaq™ for TA cloning? | |
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Yes, BlasTaq™ DNA Polymerase produces 3’-dA-tailed amplicons, which makes it compatible with TA cloning vectors.
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| Can I use BlasTaq™ for high-GC templates? | |
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Yes, BlasTaq™ is designed to handle a variety of templates, including those with high GC content. For high-GC templates, it is recommended to increase the initial denaturation step to 5 minutes to ensure complete denaturation of the template.
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| How do I know if my PCR reaction requires modification of the buffer or thermocycling conditions? | |
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BlasTaq™'s specialized buffer is optimized for most PCR applications, including primer annealing at 60°C. However, if your primers do not efficiently anneal or if you are working with particularly challenging templates, you may need to optimize the annealing temperature or adjust cycling conditions based on your results.
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| Can I store my PCR reaction mix once it has been assembled? | |
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It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
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| What is the shelf life of BlasTaq™? | |
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The shelf life of BlasTaq™ is typically indicated on the product label or datasheet, but as a general rule, it is stable for up to 1-2 years when stored properly at -20°C. Be sure to check the expiration date and make sure the enzyme remains frozen when not in use.
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| What discontinued abm product is Cat. No. G598 equivalent to? | |
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This product is abm’s next generation of PCR enzymes and is functionally equivalent to Cat. No. G011 and G039 (HotStart DNA Polymerase) as well as Cat. No. G937 (SensTaq HotStart DNA Polymerase), with improved performance. Contact our customer service team technical@abmgood.com for more information.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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