PCR Sure™ Kit
Cat. No.
G065
Unit
8 x 10 reactions
Price
$129.00
| Cat. No. | G065 | ||||||||||||||||||
| Name | PCR Sure™ Kit | ||||||||||||||||||
| Unit | 8 x 10 reactions | ||||||||||||||||||
| Category | PCR Polymerase | ||||||||||||||||||
| Description |
It has been well established that many variables can affect a PCR including template structures, inhibitors, primer design, annealing temperature, and magnesium concentration. The PCR Sure™ Kit is developed to simplify the PCR optimization process. The system consists of multiple thermostable DNA polymerases pre-mixed with 8 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up. To find the optimal conditions for your difficult PCR, all you need to do is mix templates, primers, and H2O with the PCR Sure™ 2X MasterMixes. Included in abm's PCR Buffet Program.
Additional Materials Required
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G065 |
Datasheet
| Will this work for on long template samples with 60% GC content? | |
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Yes, the PCR Sure Kit can resolve the PCR difficulties with these sort of templates. Please make sure to try the touch down protocol for a success rate.
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| The kit was supplied with a separate vial of MgCl2? Do I have to add that to the reaction? | |
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The MgCl2 is already incorporated into the reaction mix so MgCl2 does not need to be added. Very rarely, an occasional customer might want to increase the MgC12 concentration, so we included it as a courtesy.
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| I am observing positive results in my negative controls (no template added) when attempting to detect gene sequences in a bacterial strain. I suspect this may be due to residual bacterial DNA from the DNA polymerase. What steps should I take to address this issue? | |
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The presence of residual bacterial DNA is quite common in commercially available DNA polymerases, as most are expressed and purified using E. coli recombinant systems. In this case, we recommend switching to our Ultra-Pure BlasTaq™ 2X PCR MasterMix (Cat. No. G885). This product undergoes a rigorous multi-step purification protocol utilizing physical, chemical, and enzymatic methods to maximize the removal of contaminating genomic DNA.
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| How should I store this product? | |
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This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
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| Can I store my PCR reaction mix once it has been assembled? | |
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It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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