BlasTaq™ 2X qPCR MasterMix
| Cat. No. | G891 | |||||||||
| Name | BlasTaq™ 2X qPCR MasterMix | |||||||||
| Unit | 500 rxn (4 x 1.25 ml) | |||||||||
| Category | qPCR Mastermixes | |||||||||
| Description |
BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable, and robust solution for performing quantitative real-time PCR (qPCR) analysis of DNA samples. This ready-to-use MasterMix contains BlasTaq™ DNA Polymerase, a next-generation Taq polymerase strategically engineered by abm to deliver faster extension rates and stronger overall performance. With optimized reaction conditions, BlasTaq™ DNA Polymerase offers significantly improved processivity, higher yields, and enhanced sensitivity. These improvements allow for faster reaction times, shortening qPCR runs by up to 70% compared to reactions using wild-type Taq polymerase. BlasTaq™ possesses 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but lacks 3’-5’ exonuclease activity. It also produces 3’-dA-tailed amplicons, making qPCR products generated with BlasTaq™ compatible with TA cloning systems. The MasterMix contains a fluorescent dye compatible with commonly used detection systems, such as SYBR Green™ and EvaGreen™. Additionally, ROX Reference Dye is supplied separately, ensuring compatibility with most qPCR instruments. For specific instrument requirements, please refer to the ROX Machine Compatibility Chart. Product Features:
For a larger size, see Cat. No. G892 (2,500 rxn, 25.0 ml)
|
|||||||||
| Concentration | 2X | |||||||||
| Storage Condition |
Store at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
|||||||||
| Note |
BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
|
|||||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G891 |
| I still have additional questions, who do I contact? | |
|
For further details, please email technical@abmgood.com or 1-866-757-2414.
|
| Is the dye set-up similar to SYBR Green™ & EvaGreen™? | |
|
Yes, it is comparable.
|
| What are the advantages to using BlasTaq™ 2X qPCR MasterMix? | |
|
It is a ready-to-use qPCR MasterMix in which only template and primers need to be added by the end user. BlasTaq™ has super speed performance to achieve results less than a hour. ROX Reference Dye is offered on the side to give more flexibility to the user for your qPCR set up.
|
| How does BlasTaq™ differ from regular Taq polymerase? | |
|
BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
|
| How should I store this product? | |
|
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
|
| Does this have ROX reference dye? | |
|
abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
|
| How much ROX reference dye should I add to the MasterMix? | |
|
The recommended volume of ROX reference dye varies based on the qPCR instrument:
|
| Can I use this MasterMix with any qPCR machine? | |
|
Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
|
| What is the recommended amount of template DNA to use in each reaction? | |
|
Typically, 100 ng of genomic DNA is used as template in a 20 μl reaction, but the volume can be adjusted depending on the application.
|
| Can I use BlasTaq™ 2X qPCR MasterMix for miRNA cDNA templates? | |
|
Yes, BlasTaq™ 2X qPCR MasterMix can be used with miRNA cDNA templates and other appropriate applications, using the standard thermocycling conditions.
|
| What is the difference between probe-based and dye-based qPCR assays? | |
|
Probe-based assays, like those using TaqMan probes, rely on a specific probe that binds to the target DNA sequence and emits a fluorescent signal when cleaved during amplification. This provides high specificity for detecting the target sequence. Dye-based assays, like those using SYBR Green or EvaGreen, rely on a dye that binds to double-stranded DNA, emitting fluorescence as the amount of DNA increases during amplification. Dye-based assays are simpler but may be less specific since the dye binds to any double-stranded DNA, not just the target sequence. BlasTaq™ Probe 2X qPCR MasterMix is specifically designed for probe-based assays, offering higher specificity, while dye-based assays use MasterMixes like BlasTaq™ 2X qPCR MasterMix (Cat. No. G891). |
| What is the source of this enzyme? | |
|
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
|
- Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
-
Chen, L., Qin, Z., & Ruan, Z. B. (2023). Hyperoside alleviates doxorubicin-induced myocardial cells apoptosis by inhibiting the apoptosis signal-regulating kinase 1/p38 pathway. PeerJ, 11, e15315. https://doi.org/10.7717/peerj.15315
Guo, H., Chen, J., Yu, H., Dong, L., Yu, R., Li, Q., ... & Wang, W. (2023). Activation of Nrf2/ARE pathway by anisodamine (654-2) for inhibition of cellular aging and alleviation of radiation-induced lung injury. International Immunopharmacology, 124, 110864. https://doi.org/10.1016/j.intimp.2023.110864
Imtiaz, K., Farooqui, N., Ahmed, K., Zhamalbekova, A., Anwar, M. F., Nasir, A., ... & Abidi, S. H. (2025). Analysis of differential expression of matrix metalloproteinases and defensins in the nasopharyngeal milieu of mild and severe COVID-19 cases. PloS one, 20(2), e0304311. https://doi.org/10.1371/journal.pone.0304311
Jiang, K., Zhang, F., Chen, Y., Li, X., Zhao, X., Jiang, P., & Li, Y. (2024). Fosfenopril Attenuates Inflammatory Response in Diabetic Dry Eye Models by Inhibiting the TLR4/NF-κB/NLRP3 Signaling Pathway. Investigative Ophthalmology & Visual Science, 65(6), 2-2. https://doi.org/10.1167/iovs.65.6.2
Li, M., Liu, G., Jin, X. et al. Micropeptide MIAC inhibits the tumor progression by interacting with AQP2 and inhibiting EREG/EGFR signaling in renal cell carcinoma. Mol Cancer 21, 181 (2022). https://doi.org/10.1186/s12943-022-01654-1
Lin, H., Lin, J., Pan, T. et al. Polymeric immunoglobulin receptor deficiency exacerbates autoimmune hepatitis by inducing intestinal dysbiosis and barrier dysfunction. Cell Death Dis 14, 68 (2023). https://doi.org/10.1038/s41419-023-05589-3
Liu, X., Chen, Q., Yin, X., Wang, X., Ran, J., Yu, W., & Wang, B. (2024). Study on chromatin regulation patterns of expression vectors in the PhiC31 integration site. Epigenetics, 19(1), 2337085. https://doi.org/10.1080/15592294.2024.2337085
Nazim, F., Kayani, H. A., Ali Nathwani, A., Mir, F., & Abidi, S. H. (2022). CMV and EBV co-infection in HIV-infected children: infection rates and analysis of differential expression of cytokines in HIV mono-and HIV–CMV–EBV co-infected groups. Viruses, 14(8), 1823. https://doi.org/10.3390/v14081823
Ruiting, L. U. O., Huang, Y., Ruimin, B. A. I., Meng, L. I. U., Liang, S. U. N., Xiaofei, W. A. N. G., & Zheng, Y. (2024). ATP Citrate Lyase is a General Tumour Biomarker and Contributes to the Development of Cutaneous Squamous Cell Carcinoma. Acta Dermato-Venereologica, 104, 23805. https://doi.org/10.2340/actadv.v104.23805
Wang, X., Luo, X., Wang, Z., Wang, Y., Zhao, J., & Bian, L. (2023). Identification of cancer stemness and M2 macrophage-associated biomarkers in lung adenocarcinoma. Heliyon, 9(9). https://doi.org/10.1016/j.heliyon.2023.e19114
Zahid, W., Farooqui, N., Zahid, N., Ahmed, K., Anwar, M. F., Rizwan-ul-Hasan, S., … Abidi, S. H. (2023). Association of Interferon Lambda 3 and 4 Gene SNPs and Their Expression with COVID-19 Disease Severity: A Cross-Sectional Study. Infection and Drug Resistance, 16, 6619–6628. https://doi.org/10.2147/IDR.S422095
Zhang, W., Liu, J., Wang, Y. et al. Prenatal bisphenol A exposure causes sperm quality and functional defects via Leydig cell impairment and meiosis arrest in mice offspring. Sci Rep 15, 9810 (2025). https://doi.org/10.1038/s41598-025-93538-9
Zhang, X., Liu, Y., Gong, B., Wang, X., Liu, X., & Liu, F. (2025). PACAP alleviates the defective epididymis and sperm function in LPS-induced acute mice epididymitis. bioRxiv, 2025-02. https://doi.org/10.1101/2025.02.23.639790
Zhang, Y., Liu, Y., Teng, Z. et al. Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate paclitaxel-induced spermatogenesis defects and maintain male fertility. Biol Res 56, 47 (2023). https://doi.org/10.1186/s40659-023-00459-w