qPCR AAV Titer Kit
| Cat. No. | G931 | ||||||||||||||||||
| Name | qPCR AAV Titer Kit | ||||||||||||||||||
| Unit | 100 rxn | ||||||||||||||||||
| Category | qPCR Virus Titer Kits | ||||||||||||||||||
| Description |
abm’s qPCR AAV Titer Kit is a one-step assay featuring a rapid lysis step followed by qPCR, providing high sensitivity and specificity for accurate AAV titer quantification. With proprietary primer design and a pre-optimized MasterMix, this kit ensures reliable performance and outshines comparable products on the market. The included MasterMix features a fluorescent dye comparable to SYBR Green™ and EvaGreen® for optimal amplification efficiency. For maximum flexibility, the ROX Reference Dye is provided separately, ensuring compatibility with a wide range of qPCR instruments (see our ROX Machine Compatibility Chart for details). Kit Features:
Upgrade your AAV titering workflow with abm’s qPCR AAV Titer Kit—engineered for accuracy, speed, and reliability.
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| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Note |
qPCR AAV Titer Kit includes a separate vial of ROX Reference Dye. You can add this dye if your specific qPCR machine requires ROX, as indicated in the table below:
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G931 |
| What samples were used to create the standards in this kit? | |
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Plasmid with partial virus DNA were used to generate the standards.
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| Which vector elements are amplified and what is the general location of the primers? | |
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The primers target the 3'ITR region.
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| Is this kit able to detect all AAV serotypes? | |
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Yes, this kit should be able to detect all AAV serotypes. If in doubt, we can also verify for you if you can send us your ITR sequences.
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| Does this have ROX reference dye? | |
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abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
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| How much ROX reference dye should I add to the MasterMix? | |
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The recommended volume of ROX reference dye varies based on the qPCR instrument:
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| Can I use this MasterMix with any qPCR machine? | |
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Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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