qPCR AAV Titer Kit

Cat. No.

G931

Unit

100 rxn

Price

$212.00

Cat. No.

G931

Name

qPCR AAV Titer Kit

Unit

100 rxn

Category

qPCR Virus Titer Kits

Description

abm’s qPCR AAV Titer Kit is a one-step assay featuring a rapid lysis step followed by qPCR, providing high sensitivity and specificity for accurate AAV titer quantification. Designed to minimize non-specific background, the kit outperforms similar products in both reliability and performance. The MasterMix contains a dye similar to SYBR Green™ and EvaGreen®, enhancing amplification efficiency, while the ROX reference dye is provided separately to ensure compatibility with most qPCR instruments. For detailed instrument compatibility, refer to our ROX Machine Compatibility Chart.

Product Component	Quantity
BlasTaq™ 2X qPCR Titer MasterMix	1.25 ml
Primer Mix	100 rxn (200 µl)
Virus Lysis Buffer	800 µl
Standard Control DNA	50 µl
DNase I Reaction Mix	2 x 1.0 ml
ROX Reference Dye	15 µl
Nuclease-Free H₂O	2 x 1.0 ml

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Storage Condition

Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.

Note

qPCR AAV Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

MACHINE TYPE	MACHINE
High ROX Machines	ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series Biometra TOptical Fluidigm BioMark™ Wafergene SmartChip System TianLong TL998 System
Low ROX Machines	ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7 BioGene InSyte™ Illumina Eco Analytikjena qTower Series Stratagene® Mx3000, Mx3005, Mx4000
No ROX machines	BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™ Roche LightCycler® (480, 1536, Nano) MJ Research Opticon™, Opticon™ 2, Chromo® 4 Eppendorf™ Realplex 4 BioGene SynChron™ Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat) Cepheid SmartCyler, GeneXpert Enigma™ ML Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS Qiagen Rotor-Gene™ (Q, 6000) Takara Dice™ Thermo Scientific PikoReal DNA-Technology DT96, DTlite, DT-322 Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680) Bioneer Exicycler™

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G931

Datasheet

Search CoA here

MSDS

G931 MSDS

Supporting Protocol

Other

ROX Machine Compatibility

	What samples were used to create the standards in this kit?
	Plasmid with partial virus DNA were used to generate the standards.

	Which vector elements are amplified and what is the general location of the primers?
	The primers target the 3'ITR region.

	Is this kit able to detect all AAV serotypes?
	Yes, this kit should be able to detect all AAV serotypes. If in doubt, we can also verify for you if you can send us your ITR sequences.

	Does this have ROX reference dye?
	abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.

	How much ROX reference dye should I add to the MasterMix?
	The recommended volume of ROX reference dye varies based on the qPCR instrument: No ROX equipment: No dye needed. Low ROX equipment: 1 μl/1.25 ml or 22.5 μl/25 ml. High ROX equipment: 11.5 μl/1.25 ml or 225 μl/25 ml.

	Can I use this MasterMix with any qPCR machine?
	Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.

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Tanaka, Y., Kadota, S., Zhao, J. et al. Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin. Stem Cell Res Ther 14, 240 (2023). https://doi.org/10.1186/s13287-023-03468-4