qPCR Retrovirus Titer Kit
| Cat. No. | G949 | ||||||||||||||||
| Name | qPCR Retrovirus Titer Kit | ||||||||||||||||
| Unit | 100 rxn | ||||||||||||||||
| Category | qPCR Virus Titer Kits | ||||||||||||||||
| Description |
abm’s qPCR Retrovirus Titer Kit provides an advanced, simplified approach to retroviral quantification with its true one-step RT-qPCR workflow. Featuring a rapid lysis step and proprietary primer design, this kit delivers high specificity and sensitivity while significantly reducing non-specific amplification—ensuring reliable and reproducible results every time. The included MasterMix features a fluorescent dye comparable to SYBR Green™ and EvaGreen® for optimal amplification efficiency. For maximum flexibility, the ROX Reference Dye is provided separately, ensuring compatibility with a wide range of qPCR instruments (see our ROX Machine Compatibility Chart for details). Kit Features:
Achieve accurate retrovirus quantification with abm’s qPCR Retrovirus Titer Kit—designed for reliability, speed, and sensitivity.
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| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Note |
qPCR Retrovirus Titer Kit includes a separate vial of ROX Reference Dye. You can add this dye if your specific qPCR machine requires ROX, as indicated in the table below:
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G949 |
| What is the primer design based on? | |
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The primers in this kit uses a set of proprietary sequences designed based on the 5'LTR region.
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| How come my cells are not successfully infected with the evaluated titer from the kit? | |
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One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.
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| Does this have ROX reference dye? | |
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abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
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| How much ROX reference dye should I add to the MasterMix? | |
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The recommended volume of ROX reference dye varies based on the qPCR instrument:
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| Can I use this MasterMix with any qPCR machine? | |
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Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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- Colson C, Wang Y, Atherton J, Su X. SLC45A4 encodes a mitochondrial putrescine transporter that promotes GABA de novo synthesis. bioRxiv [Preprint]. 2024 Jul 24:2024.07.23.604788. https://doi.org/10.1101/2024.07.23.604788. PMID: 39091866; PMCID: PMC11291067.
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Colson C, Wang Y, Atherton J, Su X. SLC45A4 encodes a mitochondrial putrescine transporter that promotes GABA de novo synthesis. bioRxiv [Preprint]. 2024 Jul 24:2024.07.23.604788. https://doi.org/10.1101/2024.07.23.604788. PMID: 39091866; PMCID: PMC11291067.
Zhang, Y., Bassal, M., Friedrich, D., Ummarino, S., Verbiest, T., Zhang, J., ... & Tenen, D. G. (2021). C/EBPα acts as an RNA binding protein essential for its chromatin recruitment and promotes Macrophage differentiation. bioRxiv, 2021-10. https://doi.org/10.1101/2021.10.20.465166