qPCR Retrovirus Titer Kit
| Cat. No. | G949 | ||||||||||||||||
| Name | qPCR Retrovirus Titer Kit | ||||||||||||||||
| Unit | 100 rxn | ||||||||||||||||
| Category | qPCR Virus Titer Kits | ||||||||||||||||
| Description |
abm’s qPCR Retrovirus Titer Kit is a one-step assay that employs a quick RNA extraction step that is followed by RT-qPCR, providing high sensitivity and specificity for accurate retrovirus titer quantification. Designed to minimize non-specific background, the kit offers superior performance compared to other products on the market. The MasterMix contains a dye similar to SYBR Green™ and EvaGreen™, enhancing amplification efficiency, while the ROX Reference Dye is provided separately for broad compatibility with most qPCR instruments. For detailed instrument compatibility, refer to our ROX Machine Compatibility Chart.
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| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Note |
qPCR Retrovirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G949 |
| What is the primer design based on? | |
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The primers in this kit uses a set of proprietary sequences designed based on the 5'LTR region.
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| How come my cells are not successfully infected with the evaluated titer from the kit? | |
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One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.
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| Does this have ROX reference dye? | |
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abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
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| How much ROX reference dye should I add to the MasterMix? | |
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The recommended volume of ROX reference dye varies based on the qPCR instrument:
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| Can I use this MasterMix with any qPCR machine? | |
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Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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- Colson C, Wang Y, Atherton J, Su X. SLC45A4 encodes a mitochondrial putrescine transporter that promotes GABA de novo synthesis. bioRxiv [Preprint]. 2024 Jul 24:2024.07.23.604788. https://doi.org/10.1101/2024.07.23.604788. PMID: 39091866; PMCID: PMC11291067.
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Colson C, Wang Y, Atherton J, Su X. SLC45A4 encodes a mitochondrial putrescine transporter that promotes GABA de novo synthesis. bioRxiv [Preprint]. 2024 Jul 24:2024.07.23.604788. https://doi.org/10.1101/2024.07.23.604788. PMID: 39091866; PMCID: PMC11291067.
Zhang, Y., Bassal, M., Friedrich, D., Ummarino, S., Verbiest, T., Zhang, J., ... & Tenen, D. G. (2021). C/EBPα acts as an RNA binding protein essential for its chromatin recruitment and promotes Macrophage differentiation. bioRxiv, 2021-10. https://doi.org/10.1101/2021.10.20.465166