BlasTaq™ Probe 2X qPCR MasterMix
| Cat. No. | G890 | ||||||||
| Name | BlasTaq™ Probe 2X qPCR MasterMix | ||||||||
| Unit | 500 rxn (4 x 1.25 ml) | ||||||||
| Category | qPCR Mastermixes | ||||||||
| Description |
BlasTaq™ Probe 2X qPCR MasterMix is designed for TaqMan™ probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix — including dNTPs, BlasTaq™ DNA polymerase, MgCl₂, and other proprietary buffer components — have been optimized to deliver superb sensitivity and performance in under one hour. BlasTaq™ is a next-generation, strategically engineered DNA polymerase that offers faster extension rates, improved processivity, and enhanced sensitivity compared to wild-type Taq polymerase. It possesses 5’-3’ polymerase activity and 5’-3’ exonuclease activity, which is essential for TaqMan probe hydrolysis during qPCR. It does not have 3’-5’ exonuclease (proofreading) activity, and produces 3’-dA-tailed amplicons, which are compatible with TA cloning if needed. This MasterMix is suitable for a variety of applications, including SNP genotyping assays, gene expression analysis, microarray validation, and high-throughput screening. ROX reference dye is provided separately, ensuring universal compatibility with most qPCR instruments. Refer to the Rox Machine Compatibility Chart for instrument-specific guidelines. Product Features:
|
||||||||
| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
||||||||
| Note |
BlasTaq™ Probe 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
|
||||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G890 |
| Can BlasTaq™ Probe 2X qPCR MasterMix (Cat. G890) be used for multiplexing assays? | |
|
Yes, BlasTaq™ Probe 2X qPCR MasterMix (Cat. G890) can be used for multiplexing assays.
|
| Does BlasTaq™ Probe 2X qPCR MasterMix contain an antibody? | |
|
No, BlasTaq™ Probe 2X qPCR MasterMix does not contain an antibody. It includes dNTPs, BlasTaq™ DNA polymerase, MgCl₂, and proprietary buffer components, making it ideal for TaqMan probe-based real-time qPCR analysis and various applications like SNP genotyping and gene expression analysis.
|
| How does BlasTaq™ differ from regular Taq polymerase? | |
|
BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
|
| How should I store this product? | |
|
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
|
| Does this have ROX reference dye? | |
|
abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
|
| How much ROX reference dye should I add to the MasterMix? | |
|
The recommended volume of ROX reference dye varies based on the qPCR instrument:
|
| Can I use this MasterMix with any qPCR machine? | |
|
Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
|
| What is the recommended amount of template DNA to use in each reaction? | |
|
Typically, 100 ng of genomic DNA is used as template in a 20 μl reaction, but the volume can be adjusted depending on the application.
|
| What is the difference between probe-based and dye-based qPCR assays? | |
|
Probe-based assays, like those using TaqMan probes, rely on a specific probe that binds to the target DNA sequence and emits a fluorescent signal when cleaved during amplification. This provides high specificity for detecting the target sequence. Dye-based assays, like those using SYBR Green or EvaGreen, rely on a dye that binds to double-stranded DNA, emitting fluorescence as the amount of DNA increases during amplification. Dye-based assays are simpler but may be less specific since the dye binds to any double-stranded DNA, not just the target sequence. BlasTaq™ Probe 2X qPCR MasterMix is specifically designed for probe-based assays, offering higher specificity, while dye-based assays use MasterMixes like BlasTaq™ 2X qPCR MasterMix (Cat. No. G891). |
| Can I use BlasTaq™ Probe 2X qPCR MasterMix for high-throughput screening? | |
|
Yes, BlasTaq™ Probe 2X qPCR MasterMix is suitable for high-throughput screening applications, as well as other applications like SNP genotyping and gene expression analysis.
|
| What is the source of this enzyme? | |
|
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
|
- Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
-
Aydin E, Gunduz MK, Kaymak G, Sezgin AK, Dağgez H, Renders DP, Yakupoğullari Y. Antimycobacterial activity of luteolin in resistant Mycobacterium tuberculosis isolates and cytotoxicity on L929 cells. Microb Pathog. 2025 Mar;200:107287. https://doi.org/10.1016/j.micpath.2025.107287. Epub 2025 Jan 10. PMID: 39800168.
Gündüz, M. K., Kar, F., & Kaymak, G. Papain HepG2 Hücrelerinde Kaspaz-3 ve Kaspaz-9 Genlerini Düzenleyerek Apoptozu İndükler. Osmangazi Tıp Dergisi, 45(1), 94-100. https://doi.org/10.20515/otd.1181399
Koldemir Gündüz, M. BGM, a Newly Synthesised Boron Compound, Induces Apoptosis and Reduces Oxidative Stress by Inhibiting Lipogenesis in 3T3-L1 Adipocytes via PPARγ and CTRP3. Biol Trace Elem Res 200, 4807–4816 (2022). https://doi.org/10.1007/s12011-022-03261-z
Nunes, J. R., O’dwyer, C., Ghorbani, P., Smith, T. K., Chauhan, S., Robert-Gostlin, V., ... & Fullerton, M. D. (2024). Myeloid AMPK signaling restricts fibrosis but is not required for metformin improvements during CDAHFD-induced NASH in mice. Journal of Lipid Research, 65(6), 100564. https://doi.org/10.1016/j.jlr.2024.100564
Wu, J., Chen, Q., Wang, Y., Wang, R., Chen, Q., Wang, Y., ... & Chen, K. (2024). LINC01977 promotes colorectal cancer growth and metastasis by enhancing aerobic glycolysis via the ERK/c-Myc axis. Journal of Gastrointestinal Oncology, 15(1), 271. https://doi.org/10.21037/jgo-24-52
Xia, Y., Cheng, H., Bian, W., Wang, W., Zhu, M., & Zhong, J. (2022). Role of an FNIP repeat domain-containing protein encoded by Megavirus baoshan during viral infection. Journal of Virology, 96(14), e00813-22. https://doi.org/10.1128/jvi.00813-22
Zhao Z, Wang B, Jiang K, Liu M, Wang L. Comparative analysis of transcriptomics and metabolomics provides insights into the mechanisms of VPAHPND invasion and hepatopancreatic damage in Litopenaeus vannamei. Fish Shellfish Immunol. 2024 Nov;154:109920. https://doi.org/10.1016/j.fsi.2024.109920. Epub 2024 Sep 24. PMID: 39321907.