OneScript® Hot Reverse Transcriptase
Cat. No.
G593
Unit
100 rxn
Price
$160.00
| Cat. No. | G593 | ||||||||
| Name | OneScript® Hot Reverse Transcriptase | ||||||||
| Unit | 100 rxn | ||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||
| Description |
OneScript® Hot Reverse Transcriptase is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that can reverse transcribe low abundance or degraded RNA, and has significantly better resistance to contaminating inhibitors such as reagents used during RNA extraction and contaminants from biological samples. High processivity and sensitivity allow for rapid cDNA synthesis of full-length cDNA fragments in a fraction of the time of leading competitors. abm is the only company in the world to have a reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 60-72ºC. This is the enzyme of choice for daily or demanding RNA reverse transcription. OneScript® Hot is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection. Product Features:
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| Storage Condition |
Store at -20ºC. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G593 |
| Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis? | |
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Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
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| Can I synthesize cDNA from long RNA transcripts? | |
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Yes, for longer RNA transcripts, extend the 60°C incubation time to up to 30 minutes.
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| What can I use the synthesized cDNA for? | |
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The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
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| How should I store the synthesized cDNA? | |
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Store the synthesized first-strand cDNA at -20°C for long-term use.
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| Why am I getting low cDNA yields? | |
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Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
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| How much RNA template should I use for cDNA synthesis? | |
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We recommend using 1 ng to 2 μg of RNA per reaction.
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| What volumes of cDNA should I use for downstream PCR? | |
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Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
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What if input RNA samples are expected to have high levels of genomic DNA contamination?
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If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).
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| What is the advantage of OneScript® Hot Reverse Transcriptase compared to OneScript® Plus Reverse Transcriptase? | |
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OneScript® Hot Reverse Transcriptase offers a significant advantage over enzymes that operate at lower temperatures (42-55ºC) due to its exceptional thermostability, allowing it to function effectively at 60-72ºC. This higher temperature improves efficiency by preventing RNA secondary structures and enabling cDNA synthesis from degraded or low-abundance RNA. Additionally, it is more resistant to inhibitors and contaminants, making it ideal for complex or impure RNA samples.
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Do I need an RNase inhibitor when using this product?
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All of abm's Reverse Transcriptases include RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation and is resistant to oxidation, even under low DTT concentrations. Therefore, there’s no need to add an external RNase inhibitor, as RNaseOFF provides optimal RNA protection during reverse transcription.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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Mariano, A., Ammendola, S., Migliorini, A., Leopizzi, M., Raimondo, D., & Scotto d'Abusco, A. (2024). Intron retention in PI‐PLC γ1 mRNA as a key mechanism affecting MMP expression in human primary fibroblast‐like synovial cells. Cell Biochemistry and Function, 42(5), e4091. https://doi.org/10.1002/cbf.4091
Zhang, Y., Zheng, Z., Gao, J., Bao, X., Zhang, W., Liu, L., ... & Li, Y. (2024). Rare-Earth Metal-Based Nanosystems for Facilitating Neural Stem Cell Differentiation into Neurons and Enhancing Axonal Stability. ACS Applied Nano Materials, 7(14), 16154-16161. https://doi.org/10.1021/acsanm.4c02057
Pietrangelo, T., Santangelo, C., Bondi, D., Cocci, P., Piccinelli, R., Piacenza, F., ... & Palermo, F. (2023). Endurance-dependent urinary extracellular vesicle signature: shape, metabolic miRNAs, and purine content distinguish triathletes from inactive people. Pflügers Archiv-European Journal of Physiology, 475(6), 691-709. https://doi.org/10.1007/s00424-023-02815-x
Camas Carangui, L. I., & Chabla Morquecho, N. S. (2024). Efecto del ciclo lunar en algunas características morfológicas y funcionales del ovario en cobayas (Cavia porcellus). http://dspace.ucuenca.edu.ec/handle/123456789/44942
Gutiérrez Tacuri, D. T., & Lucero Zari, G. T. (2024). Expresión génica del factor de necrosis tumoral alfa y del receptor de melatonina en células del bazo de cobayas en cuatro momentos del ciclo lunar. https://dspace.ucuenca.edu.ec/handle/123456789/44989
Sun, Z., Geng, W., Ren, B., Zhao, B., Liu, P., & Zhang, J. (2022). Low photosynthetic rate under low light stress inhibited sucrose distribution and transportation to grain. BioRxiv, 2022-08. https://doi.org/10.1101/2022.08.02.502494
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