saCas9 Null Mutant Protein

Cat. No.

K146

Unit

32.5 µg (250 pmol/25 µL)

Price

$162.00

Cat. No.

K146

Name

saCas9 Null Mutant Protein

Unit

32.5 µg (250 pmol/25 µL)

Category

Cas Proteins & CRISPR Screening

Description

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.

The saCas9 Null Mutant Protein is created by mutating both cleavage domains of the wild type saCas9. Such a saCas9 protein retains its ability to bind to genomic DNA through sgRNA:genomic DNA base pairing, however, the saCas9 Null Mutant does not induce cleavage. Therefore, this protein can provide a useful negative control for CRISPR experiments. In addition, binding of the Null Mutant can act as a roadblock to hinder transcription, thus offering a useful tool to achieve reversible knock-down of gene expression.

The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).

abm offers a variety of Cas9 proteins. View our whole collection here.

Cas Type

Null Mutant

Cas Origin

saCas9

Cas Protein Marker

No GFP

Concentration

10 µM, 1.30 mg/ml

Format General

Enzyme supplied with 10X Reaction Buffer

Endotoxin Level

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Storage Buffer

10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.

Storage Condition

Store all components at -20°C.

Caution

This product is distributed for laboratory research only. Not for diagnostic use.

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K146

Datasheet

saCas9 Null Mutant Protein Datasheet

Search CoA here

MSDS

Cas9 Proteins MSDS

	Does K146 contain a HIS-tag?
	Yes, it does contain a His-tag.

	What is the source of this enzyme?
	The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.