pAAV-G-CMV
| Vector Name | pAAV-G-CMV |
| VectorType | AAV Vector |
| Antibiotic Information | Bacterial: Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` AAV insert reverse primer 5`---CAC TGC ATT CTA GTT GTG GTT TGT CC---3` |
| Additional Information | The gene expression is driven by a CMV promoter, without any Tag or reporter. Original Vector: 6409bp |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctagggt
661 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
721 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
781 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
841 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
901 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtttag tcatcgctat
961 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
1021 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
1081 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
1141 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
1201 acgccatcca cgctgttttg acctccatag aagaaccgag tttaaactcc ctatcagtga
1261 tagagatctc cctatcagtg atagagagct agcgatatca acaagtttgt acaaaaaagc
1321 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
1381 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
1441 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
1501 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
1561 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
1621 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
1681 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
1741 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
1801 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
1861 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
1921 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
1981 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
2041 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
2101 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
2161 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc
2221 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
2281 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
2341 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
2401 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
2461 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
2521 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
2581 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
2641 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
2701 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
2761 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
2821 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
2881 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
2941 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
3001 gtggttgata tctgactcga gtacccatac gacgtcccag actacgctac gcgtcttaag
3061 gcgatcgcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
3121 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
3181 agctgcaata aacaagttaa taacaacaat tccattcatt ttatgtttca ggttcagggg
3241 gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtag tcgaaattcc
3301 cgataaggat cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta
3361 actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
3421 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
3481 gcgagcgagc gcgcagggat cccggtgtga aataccgcac agatgcgtaa ggagaaaata
3541 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
3601 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
3661 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
3721 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3781 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3841 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3901 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3961 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
4021 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
4081 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
4141 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
4201 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
4261 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
4321 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
4381 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
4441 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
4501 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
4561 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
4621 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
4681 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
4741 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
4801 tgaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
4861 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
4921 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
4981 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
5041 aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg atcaagctct
5101 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
5161 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
5221 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
5281 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
5341 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
5401 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
5461 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
5521 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
5581 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
5641 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
5701 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
5761 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
5821 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
5881 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt
5941 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaacatcc cttataaatc
6001 aaaagaatag accgcgatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
6061 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
6121 acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag ctctaaatcg
6181 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
6241 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
6301 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc
6361 cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
LOCUS dna 6409 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 837..842
/dbxref="REBASE:NdeI"
Promoter 1111..1192
/gene="CMV prom"
misc_binding 1167..1172
/dbxref="REBASE:SacI"
Regulatory_Seq 1248..1287
/gene="tetO reg"
Other Gene 1301..1425
/gene="attR1 other"
Other Gene 1325..1425
/gene="attR2 other"
misc_binding 1426..1433
/dbxref="REBASE:NotI"
Marker 1534..2193
/gene="CAT marker"
ORF 1534..2193
/sequence="ORF_1 rf(1)"
misc_binding 1747..1752
/dbxref="REBASE:EcoRI"
Other Gene 2535..2840
/gene="ccdB other"
misc_binding 2869..2874
/dbxref="REBASE:PstI"
Other Gene 2881..3000
/gene="attR1 other"
Other Gene 2881..2981
/gene="attR2 other"
misc_binding 3016..3021
/dbxref="REBASE:XhoI"
Rep_Origin 3721..4340
/gene="pBR322 origin"
Promoter 4993..5042
/gene="NEOKAN prom"
misc_binding 5100..5105
/dbxref="REBASE:BclI"
ORF 5131..5925
/sequence="ORF_2 rf(1)"
Marker 5134..5922
/gene="NTP_II marker"
misc_binding 5660..5665
/dbxref="REBASE:SphI"
Rep_Origin 6030..6335
/gene="f1 origin"