Collagenase Type I

Cat. No.
G7201
Unit
100 mg
Price
$42.00
Cat. No. G7201
Name Collagenase Type I
Description

Collagenase Type I is a proteolytic enzyme preparation derived from Clostridium histolyticum, specifically targeting native collagen and efficiently degrading interstitial collagens (Types I, II, III, and IV). It is widely used in primary cell isolation, tissue dissociation, and extracellular matrix (ECM) remodeling studies.

This enzyme is particularly effective for isolating fibroblasts, hepatocytes, epithelial cells, and other primary cells from tissues by breaking down collagen-rich connective structures, while maintaining high cell viability and functionality.

Key Features:

  • Derived from Clostridium histolyticum

  • Targets native collagens (I, II, III, IV)

  • Optimized for tissue dissociation and primary cell isolation

  • High activity and consistent lot-to-lot performance

  • Supplied as lyophilized powder for convenience and stability

 

Applications:

  • Isolation of primary cells from tissues

  • Preparation of single-cell suspensions

  • ECM and collagen degradation studies

  • Tissue engineering and regenerative medicine workflows

 

Specifications:

  • CAS No.: 9001-12-1

  • Form: Lyophilized powder

  • Activity: ≥125 U/mg

  • Recommended Reconstitution: Sterile PBS or culture medium (e.g., 1–2 mg/mL)

Unit 100 mg
Usage

An enzyme that digests native collagen fibrils by cleaving peptide bonds in the collagen triple helix. Commonly used for tissue dissociation to release primary cells or iPSCs.

Storage Condition Lyophilized powder stable at 2–8 °C (short-term) or -20 °C (long-term).
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G7201
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