Column-Pure Plasmid Miniprep Kit
| Cat. No. | G4003 |
| Name | Column-Pure Plasmid Miniprep Kit |
| Unit | 250 Preparations |
| Description |
abm’s Column-Pure Plasmid Miniprep Kit utilizes the standard alkaline lysis method with silica spin column technology to rapidly extract and purify plasmid DNA from cells. The extracted high quality plasmid DNA is primarily in supercoiled form and is ready for downstream applications such as PCR, restriction digest and sequencing. Principle: The procedure involves an alkaline lysis method to break down bacterial cells, followed by DNA adsorption onto silica in the presence of high salt. The unique silica membrane replaces traditional glass or silica slurries, streamlining the plasmid DNA preparation. The process includes three steps: 1) bacterial lysate preparation and clearing via alkaline lysis, 2) transfer of the supernatant to a column for DNA binding, and 3) washing away proteins and impurities, followed by nucleic acid elution with a low-salt buffer. Key Features:
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| Storage Condition |
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37°C to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G4003 |
| What is the binding capacity of the miniprep columns? | |
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Up to 35 µg plasmid DNA from 1-5 ml of bacterial culture.
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