Anti-GAPDH Loading Control Monoclonal Antibody
Cat. No. | G041 |
Name | Anti-GAPDH Loading Control Monoclonal Antibody |
Category | Loading Control Antibodies |
Application | WB,ICC/IF |
Raised In | Mouse |
Clonality | Monoclonal |
Reactivity | Other |
Description | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
Isotype | IgG |
Concentration | 100ug/100ul |
Recommended Dilutions | It is very useful as a loading control for quantitative western blotting. The antibody recognizes a 37kDa band in a Western Blot analysis. |
Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
Formulation | Liquid |
Guarantee | abm guarantees that all our Anti-GAPDH Loading Control Monoclonal Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G041 |
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I am working with Dictyostelium discoideum, a cellular slime mould. I was wondering if this antibody could work in dictyostelium? | |
If the sample was processed properly, the sample expresses GAPDH, and the GAPDH is highly homologous to the human version, then it is likely this antibody will work on your sample.
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