Cas9 Nuclease Protein
| Cat. No. | K108 | |
| Name | Cas9 Nuclease Protein | |
| Unit | 40 µg (250 pmol/25 µL) | |
| Category | Cas Proteins & CRISPR Screening | |
| Description |
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.
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| Cas Type | Nuclease | |
| Cas Origin | spCas9 | |
| Cas Protein Marker | No GFP | |
| Concentration | 10 µM, 1.60 mg/ml | |
| Format General | Enzyme supplied with 10X Reaction Buffer | |
| Storage Buffer | 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol. | |
| Storage Condition | Store all components at -20°C. | |
| Caution | This product is distributed for laboratory research only. Not for diagnostic use. | |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K108 |
| Does this cas9 nuclease protein contain a Nuclear Localization Signal? | |
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No, this Cas9 nuclease protein does not have a Nuclear Localization Signal and therefore represents the native form. If NLS is desired, we would recommend our Cas9 Nuclease NLS Protein (Cat#K030/K130). A full list of our Cas9 products can be found here:
https://www.abmgood.com/CRISPR-Cas9-Nuclease-Nickase-Vectors-Viruses.html
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| Does the cas9 nuclease protein contain a HIS-tag? | |
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Yes, it does contain a His-tag.
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| Does K108 contain a HIS-tag? | |
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Yes, it does contain a His-tag.
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| Does this cas9 nuclease protein contain a Nuclear Localization Signal? | |
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No, this Cas9 nuclease protein does not have a Nuclear Localization Signal and therefore represents the native form. If NLS is desired, we would recommend our Cas9 Nuclease NLS Protein (Cat#K030/K130). A full list of our Cas9 products can be found here:
https://www.abmgood.com/CRISPR-Cas9-Nuclease-Nickase-Vectors-Viruses.html
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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- Bláhová, Z., Franěk, R., Let, M., Bláha, M., Pšenička, M., & Mráz, J. (2022). Partial fads2 Gene Knockout Diverts LC-PUFA Biosynthesis via an Alternative Δ8 Pathway with an Impact on the Reproduction of Female Zebrafish (Danio rerio). Genes, 13(4), 700. https://doi.org/10.3390/genes13040700