Peroxidase Conjugated Affinity Purified anti-Golden Syrian Hamster IgG [H&L] [Goat]
Cat. No. | SH015 |
Name | Peroxidase Conjugated Affinity Purified anti-Golden Syrian Hamster IgG [H&L] [Goat] |
Category | Secondary Antibodies |
Reactivity | Hamster |
Immunogen | Golden Syrian Hamster IgG, whole molecule |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Recommended Dilutions | This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:25,000 to 1:125,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher. |
Storage Buffer | 0.01% (w/v) Gentamicin Sulfate |
Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Golden Syrian Hamster IgG and Golden Syrian Hamster Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. Greatly reduced reactivity will occur against Armenian Hamster IgG. |
Stabilizer | 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free |
Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. SH015 |
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