Random Primer (6N)
Cat. No.
G139
Unit
500 μl, 10 μM
Price
$45.00
| Cat. No. | G139 |
| Name | Random Primer (6N) |
| Category | Buffers & Chemicals |
| Description |
This product contains 500 μl of 10 μM Random Primer (6N) of this primer, dissolved in ddH2O. |
| Recommended Protocol | This product is intended for use in reverse transcription cDNA synthesis reactions, and should be added to reactions at a final concentration of 0.5-1 μM. |
| Storage Condition |
This product can be stored at -20°C for a maximum of 60 months. Avoid freeze-thaw cycles. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G139 |
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| What is the molecular weight of this primer? | |
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The molecular weight of the primer can be calculated as follow (it is just 5'-NNNNNN-3'):
MW = (dA = 314.21g/mol) + (dC = 289.18g/mol) + (dG = 329.21g/mol) + (dT = 304.19g/mol)/4 x 6 – 62.97 + 1.008
The weight must be corrected by subtracting 62.97, which is equal to two oxygen atoms and a phosphorus atom, to account for the 5’-terminal OH group, and by adding 1.008, which is equal to one hydrogen atom, to account for the 3’-terminal OH group.
Please note that this would be just the average MW of the N6 random primer.
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