Recombinant Human BCA-1 (CXCL13)

Cat. No.

Z100075

Unit

Price

$320.00

Cat. No.	Z100075
Name	Recombinant Human BCA-1 (CXCL13)
Description	The homeostatic chemokine CXCL13 is constitutively expressed in secondary lymphoid tissue and known to initiate lymphoid neogenesis when expressed aberrantly in mice. CXCL13 has also been detected in chronic inflammation associated with human lymphoid neogenesis, suggesting a pathogenic role. Follicular dendritic cells are generally considered to be the major source of CXCL13 both in normal and aberrant lymphoid tissue. CXCL13 has been the exclusive ligand for CXCR5, which initiates chemotaxis toward an increasing gradient in vitro and to B cell zones in lymph nodes that express CXCL13. More recently, CXCR5 expression has also been shown in DN T cells in mice, a subset of CD4 follicular Th cells, and some tonsillar CD4+ and CD25+ Tregs in humans.
Organism	Human (H. sapiens)
Unit quantity	20 µg
Family	Chemokine-alpha Family (CXC)
Aliases	ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13
Gene Symbol	CXCL13
Gene ID	10563
Accession Number	O43927
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	10 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS,100 mM NaCl, pH 7.5
Function	Determined by its ability to chemoattract human CXCR5 transfected mouse BaF3 B cells, the ED(50) is less than 20 ng/mL
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100075

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.