Recombinant Human BMP-3 beta (GDF10)
| Cat. No. | Z100095 |
| Name | Recombinant Human BMP-3 beta (GDF10) |
| Description |
BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP3 is a glycoprotein that is identical with osteogenin. Human and rat mature proteins are 98% identical. |
| Organism | Human (H. sapiens) |
| Unit quantity | 20 µg |
| Family | TGF-beta Super-family |
| Aliases | BIP, BMP-3b, BMP3B |
| Gene Symbol | GDF10 |
| Gene ID | 2662 |
| Accession Number | P55107 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 13 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity |
>95% as determined by SDS-PAGE |
| Formulation | Recombinant BMP-3b was lyophilized from a 0.2 μm filtered 1.0 mg/mL 10 mM sodium citrate (pH 3.5) containing 10% glycerol. |
| Function | Not available. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycle. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100095 |
| How do I choose the right growth factor for my experiment? | |
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| What is the shelf life and storage condition of this product? | |
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The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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