Recombinant Human CD40 Ligand (CD40LG) (E. coli)

Cat. No.

Z100125

Unit

Price

$92.00

Cat. No.	Z100125
Name	Recombinant Human CD40 Ligand (CD40LG) (E. coli)
Description	CD40LG protein is expressed on all B lymphocytes during various stages of development, activated T cells and monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. It is expressed on most mature B cell malignancies and on some early B cell acute lymphocytic leukemias. Human CD40LG has been found to be the receptor for a protein involved in an X-linked immunodeficiency syndrome. Based on its homology with other members of the TNF ligand protein superfamily, CD40LG is also referred to also as TNFSF5. The soluble form of CD40LG is generated by the intracellular proteolytic processing of the full length CD40LG. Recombinant Human CD40LG is a 17 kDa protein that represents the soluble segment of full length CD40LG and encompasses the receptor binding TNF-like domain.
Organism	Human (H. sapiens)
Unit quantity	50 µg
Family	TNF Super-family
Aliases	CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Gene Symbol	CD40LG
Gene ID	959
Accession Number	P29965
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	16 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.0)
Function	The ED50 as determined by dose-dependent proliferation of acute myeloid leukemia was found to be <5 ng/ml
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100125

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Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
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Concentration and formulation: Different experiments may require varying concentrations of growth factors.

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.