Recombinant Human GDF3 (E. coli)

Cat. No.

Z101735

Unit

Price

$290.00

Cat. No.	Z101735
Name	Recombinant Human GDF3 (E. coli)
Description	GDF-3 is a growth and differentiation factor which belongs to the TGF-beta superfamily. GDF-3 and GDF-9 are the only two members of the family which do not form disulfide-linked dimers. GDF-3 has been found in adult bone marrow, spleen, thymus, and adipose tissue. Although signaling in all other members of the family involve the BMP receptors ALK1,2,3, and 6, GDF-3 signals through BMP receptors ALK4 and 7.
Organism	Human (H. sapiens)
Unit quantity	20 µg
Family	TGF-beta Super-family
Tag	Tag Free
Aliases	KFS3, MCOP7, MCOPCB6
Gene Symbol	GDF3
Gene ID	9573
Accession Number	Q9NR23
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	13 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from solution in 30mM Acetate buffer, pH 4
Function	Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells.
Reconstitution	A quick spin of the vial followed by reconstitution in sterile 100mM Acetate buffer, pH 4 at a concentration of 0.5 mg/mL. Please note that in higher concentrations the solubility of GDF3 is limited. The protein is not lypholized in a filtered solution. Please sterile filter before using it in the cell culture.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101735

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MSDS

Growth Factors, Cytokines & Kinases MSDS

Other

How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.