Recombinant Human IGF2 (E. coli)

Cat. No.

Z102345

Unit

Price

$54.00

Cat. No.	Z102345
Name	Recombinant Human IGF2 (E. coli)
Description	Insulin-like growth factor 2 (IGF2) is one of the mitogenic, polypeptide growth factors that belongs to the insulin family, due to its structural and functional similarity to insulin. IGF2 is mainly produced in the liver and several other tissues at certain times. IGF2 expression can be found in many tissues usually with autocrine, paracrine and endocrine functions. IGF2 mainly acts as a growth promoting hormone during gestation, and does so by binding to the tyrosine kinase type I receptor (IGF1R) as well as the IGF2 receptor cation-independent mannose 6-phosphate receptor. Mature IGF2 is highly conserved between human, bovine, and porcine species. Recombinant Human IGF2 is a single, non-glycosylated, polypeptide chain of 7.8 kDa.
Organism	Human (H. sapiens)
Unit quantity	10 µg
Family	IGF/Insulin Family
Aliases	C11orf43, GRDF, IGF-II, PP9974, SRS3
Gene Symbol	IGF2
Gene ID	3481
Accession Number	P01344
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	7.8 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in Tris and NaCl
Function	The ED50 as determined by dose dependent proliferation of MCF7 cells was <1ng/ml
Reconstitution	Reconstitute in sterile PBS at 100 µg/mL
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102345

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.