Recombinant Human MIP-3 beta (CCL19) (E. coli)

Cat. No.

Z101945

Unit

Price

$290.00

Cat. No.	Z101945
Name	Recombinant Human MIP-3 beta (CCL19) (E. coli)
Description	CCL19 is a chemokine that belongs to the CC family of growth factors and signals through the CCR7 receptor. CCl19 has been shown to be expressed in the thymus, lymph nodes and in activated bone marrow stromal cells. When anti-inflammatory signals are released, expression of CCL19 gets down-regulated – specifically by IL-10.
Organism	Human (H. sapiens)
Unit quantity	20 µg
Family	Chemokine-beta Family (CC)
Aliases	CKb11, ELC, MIP-3b, MIP3B, SCYA19
Gene Symbol	CCL19
Gene ID	6363
Accession Number	Q99731
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	9.1 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution containing no additives
Function	Measured by its ability to chemoattract 5 10 day cultured human peripheral blood lymphocytes (PBL). The ED(50) for this effect is typically 0.10.3 µg/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101945

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.