Recombinant Human THPO

Cat. No.

Z101515

Unit

Price

$290.00

Cat. No.	Z101515
Name	Recombinant Human THPO
Description	Thrombopoietin, known also as TSF (thrombopoiesis stimulating factor), for mediator substances that function as natural stimulators of megakaryocytopoiesis and thus, promote the differentiation of platelets. Thrombopoietin stimulates an increase in the sizes and numbers of megakaryocytes. It also increases the number of small acetyl-cholinesterase positive cells that are early precursor cells of the megakaryocytic lineage. Thrombopoietin is a protein of 332 amino acids that shows significant homology with Epo (erythropoietin) in its N-terminal domain. A comparison of human and porcine Thrombopoietin sequences shows 83 % identity between the erythropoietin-like domains but only 67 % between the C- terminal domains.
Organism	Human (H. sapiens)
Unit quantity	10 µg
Family	HDGF/S-100/EPO(TPO) Families
Aliases	CAMT2, MGDF, MKCSF, ML, MPLLG, THC9, THCYT1, TPO
Gene Symbol	THPO
Gene ID	7066
Accession Number	P40225
Source	HEK293 cells
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	35 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	Greater than 95% as observed by SDS-PAGE.
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.0)
Function	The ED50 as determined by the dose-dependent proliferation of Mo7e cells was found to be in the range of 1-2 ng/mL
Reconstitution	It is recommended to reconstitute the lyophilized Thrombopoietin in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Storage	Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101515

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.