Recombinant Mouse MCP-2 (CCL8) (E. coli)

Cat. No.

Z200395

Unit

Price

$92.00

Cat. No.	Z200395
Name	Recombinant Mouse MCP-2 (CCL8) (E. coli)
Description	Monocyte chemotactic protein (MCP-2) is a C-C chemokine. It shares over 60% amino acid identity with MCP-1 and MCP- 3 and has about 30% identity with other C-C chemokines MIP-1 alpha, RANTES, and MIP-1 beta. MCP-2 is chemotactic for and activates a wide variety of inflammatory cells. Also, its spectrum of action on leukocytes is similar to that of MCP-3. The leukocytes affected are monocytes, T lymphocytes, natural killer cells, basophils, mast cells, and eosinophils - differs from MCP-1 which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP-1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells.
Organism	Mouse (M. musculus)
Unit quantity	20µg
Family	Chemokine-beta Family (CC)
Tag	Tag Free
Aliases	1810063B20Rik, HC14, MCP-2, Mcp2, Scya8
Gene Symbol	CCL8
Gene ID	20307
Accession Number	Q9Z121
Source	E. coli
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	9 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Mouse MCP-2 was lyophilized from a 0.2 μm filtered 20 mM PB solution pH 7.5, 100 mM NaCl.
Function	The ED(50) was determined by the dose-dependent chemo-attraction human PBMC using a range of 10-100 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200395

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.