Recombinant Mouse SHH (E. coli)

Cat. No.

Z200615

Unit

Price

$113.00

Cat. No.	Z200615
Name	Recombinant Mouse SHH (E. coli)
Description	Mouse SHH is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. Mouse SHH shares 99% homology with the human gene for SHH. Sonic HedgeHog is a protein that is vital in guiding the early embryo; it has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. SHH binds to the patched receptor to activate the transcription of target genes. Sonic HedgeHog also regulates another factor, the gli oncogene. Recombinant Mouse SHH is a single, non-glycosylated polypeptide chain of 19.8 kDa.
Organism	Mouse (M. musculus)
Unit quantity	25 µg
Family	Hedgehog Family
Aliases	9530036O11Rik, Dsh, HHG-1, Hhg1, Hx, Hxl3, M100081, ShhNC
Gene Symbol	SHH
Gene ID	20423
Accession Number	Q62226
Source	E. coli
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	19.8 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in phosphate buffer and NaCl
Function	The ED50 of this protein, as measured by its ability to induce alkaline phosphatase production in mouse myoblast cell, was in the range of 500ng-3 ug/mL.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200615

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Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.