saCas9 Nuclease NLS Protein (Lyophilized)
| Cat. No. | K152 | |
| Name | saCas9 Nuclease NLS Protein (Lyophilized) | |
| Unit | 32.5 µg (250 pmol) | |
| Category | Cas Proteins & CRISPR Screening | |
| Description |
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR). The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT). saCas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. This saCas9 Nuclease NLS protein is offered as lyophilized powder for better long term storage and ambient temperature shipping. The protein is lyophilized as a low-salt powder, so it can be resuspended in your desired buffer for downstream applications.
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| Cas Type | Nuclease | |
| Cas Origin | saCas9 | |
| Cas Protein Marker | No GFP | |
| Format General | Lyophilized powder. Enzyme supplied with 10X Reaction Buffer. | |
| Endotoxin Level | <1.0 EU/μg of recombinant protein as determined by the LAL method. | |
| Storage Buffer | After resuspension in 25 μl nuclease-free dH2O: 20mM HEPES, pH 7.5, 100mM KCl, 1mM DTT, 0.1mM EDTA, 1% sucrose. | |
| Storage Condition | Store all components at -20°C. | |
| Caution | This product is distributed for laboratory research only. Not for diagnostic use. | |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K152 |
| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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