1kb Plus Opti-DNA Marker
Cat. No.
G248
Unit
500 μl/100 loads
Price
$49.00
| Cat. No. | G248 | ||||||||
| Name | 1kb Plus Opti-DNA Marker | ||||||||
| Category | DNA & Protein Ladders | ||||||||
| Description |
PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 14 bands, ranging from 250 pb – 25 kb, for molecular weight standards in agarose gel electrophoresis. The 1,000 and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size. Total DNA concentration for all bands combined is 103.2ng/μl.
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| Concentration | 104 μg/ml | ||||||||
| Application |
For approximating DNA size and amount on agarose gels. |
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| Storage Condition |
Store @ 4°C for up to 3 months or -20°C for up to 24 months. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G248 | ||||||||
Print/Download Datasheet
| How much of the should I load onto the gel? | |
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It is recommended to gently mix the ladder by pipetting up and down a few times, then load 5 μl directly into the well of the gel.
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| Can I estimate the DNA mass in my sample using this ladder? | |
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Yes. The DNA ladder includes approximated mass values for each band (based on a 5 μl load), allowing you to estimate the mass of DNA in samples with comparable intensity and size.
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| Which bands have increased intensity and why? | |
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The 1,000 bp and 3,000 bp bands are of increased intensity, making them ideal as quick visual reference points when interpreting gel results.
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