SafeView™ Classic

Cat. No.

G108

Unit

1.0 ml

Price

$78.00

Cat. No.	G108
Name	SafeView™ Classic
Unit	1.0 ml
Category	Gel Documentation
Description	abm’s SafeView™ Classic is a safe, improved formulation of nucleic acid stain for agarose gel electrophoresis. SafeView™ Classic directly replaces toxic Ethidium Bromide and eliminates its associated contamination risks with glassware, gel apparatus and environment. Features: •Easy to Use: 10,000X stock and compatible with both UV and blue light. •Safe: Non-carcinogenic. •Sensitive: Detect as little as 1-2 ng of DNA per gel band. •Stable: Store at room temperature (18-25°C). •Superior: Compatible with downstream applications such as cloning and sequencing.
LED Viewer Compatibility	Yes
Application	Safe Detection of dsDNA, ssDNA and RNA in agarose gels.
Concentration	10,000X
Format General	Pre-cast or Post-stain
Storage Condition	Store at 18-25°C
Note	Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G108

Datasheet

SafeView™ Classic Datasheet

Search CoA here

MSDS

SafeView™ Classic MSDS

Other

SafeViewER-Flyer

	Can SafeView products be used in replacement of ethidium bromide?
	All SafeView™ products (Safe-Green™, Safe-Red™, SafeView™ Classic, Safe-Red™ Gel) can be used as a complete replacement for your ethidium bromide workflow.

How do SafeView products work and why are they not carcinogenic?

SafeView™ products contain fluorescent compounds that have a strong affinity to nucleic acids. Once bound to a nucleic acid, the compound fluoresces under specific wavelength of light which can then be visualized using a standard UV/Blue light imager. There may be some unknown effects of SafeView™ products that have not been documented in literature but these would also apply to equivalent popular products such as SYBRSafe. However, SafeView™ products are not as carcinogenic as ethidium bromide.

How do I use SafeView products?

Safe-Red™ and Safe-Green™ are supplied in a 6X loading dye format: mix samples and DNA marker with Safe-Red™ or Safe-Green™ at a 1:5 (dye : sample) dilution ratio, and load onto an unstained gel.

SafeView™ Classic and Safe-Red™ Gel are supplied in a 10,000X format: for pre-cast, add 10µl SafeView™ Classic or Safe-Red™ Gel per 100ml molten agarose, mix gently and cast the gel; for post-stain, prepare and run an unstained agarose gel, next submerge the gel in post-staining solution of 30μl SafeView™ Classic or Safe-Red™ Gel per 100ml of 1X TAE or 1X TBE buffer for 30min in the dark with light agitation, then image accordingly.

	Can SafeView products differentiate between double stranded and single stranded nucleic acids?
	No, our SafeView products will bind to both double-stranded and single-stranded nucleic acids, albeit with lesser efficiency for single-stranded nucleic acid species.

	At what temperature do I store SafeView products?
	SafeView Classic™, Safe-Red™ and Safe-Red™ Gel should be stored at 18-25°C. Safe-Green™ should be stored at 4°C.

How long can a SafeView Classic stained gel be stored for?

We recommend imaging a SafeView™ Classic stained gel immediately after it has finished running in order to maintain optimal brightness, sensitivity and band sharpness. Prolonged storage of a used gel may result in decreased sensitivity and diffusion of bands. A pre-cast unused SafeView™ Classic gel can be stored and used up to 1 week after being prepared provided they are stored in ideal conditions e.g. sealed in plastic bag away from light. If stored for longer than 1 week, there will be decreased resolution.

	How sensitive are SafeView products?
	Safe-Green™: 0.2-0.6 ng DNA per band Safe-Red™: 0.6-1.0 ng DNA per band Safe-Red™ Gel: 0.6-1.0 ng DNA per band SafeView™ Classic: 1.0-2.0 ng DNA per band

	Can SafeView products be used to post-stain gels?
	Yes, SafeView™ Classic and Safe-Red™ Gel can both be used for either pre-cast or post-stain gels.

How do I dispose of used gloves, electrophoresis buffer and agarose gels stained with SafeView?

Dispose of SafeView™ Classic as you would any other non-carcinogenic fluorescent dye (e.g. Acridine orange, Propidium iodide). All gels and contaminated “non-sharp” lab debris (e.g., gloves, pads, towels, tubes, etc.) that are processed using this stain can be discarded in regular landfill trash. Used electrophoresis buffer and staining solutions that contain the stain can either be collected and disposed of through the HWMU or collected and run through an approved filter device. The buffer solutions that have been run through the approved filter should be checked under the appropriate light source for complete removal of the dyes, and if it passes (does not fluoresce), the liquid can be disposed of down the drain with a copious amount of water as long as no other materials are present that would cause the material to be a Hazardous Waste. The filters that have been used up and are no longer effective must be disposed of through the HWMU.

	Do I need to add any loading dye to my samples prior to running them on a SafeView agarose gel?
	If using Safe-Green™ or Safe-Red™, you will not need to add any additional loading dyes to your samples. If using SafeView™ Classic or Safe-Red™ Gel, you will need to add an appropriate DNA loading dye to your samples in order for samples to properly settle and stay in the wells during electrophoresis.

	I cannot visualize 100bp and 200bp DNA bands on a 1% SafeView agarose gel. What should I do?
	It is often difficult to detect smaller 100bp and 200bp bands on a 1% gel especially if samples are of low concentration. We recommend visualizing smaller fragments on a higher concentration gel - ideally 2% agarose.

	Can I use SafeView products to visualize nucleic acids on a polyacrylamide gel?
	SafeView™ products are designed for use with agarose gels, and are not optimized for polyacrylamide gels. However, SafeView™ Classic and Safe-Red™ Gel can both visualize nucleic acids on polyacrylamide gels (Acrylamide, TBE, APS, TEMED) by post staining method.

	Can SafeView products be stored at -20°C?
	No, please store Safe-Green™ at 4°C and SafeView™ Classic, Safe-Red™ and Safe-Red™ Gel at 18-25°C.

	What is the concentration of SafeView products?
	SafeView™ Classic and Safe-Red™ Gel are supplied at a 10,000X concentration. Safe-Green™ and Safe-Red™ are supplied at a 6X concentration.

	Can SafeView Classic stain penetrate cell membranes?
	Yes, SafeView™ Classic can penetrate living cell membranes.

	Can I perform gel extraction and subsequent cloning using SafeView products?
	We recommend using SafeView™ Classic or Safe-Red™ Gel which can both be used for gel extraction of DNA fragments destined for cloning. We have observed no decrease in cloning efficiency in comparison to ethidium bromide.

	What type of device or imaging system should be used to visualize SafeView agarose gels?
	We recommend using abm’s SafeViewER™ Imager (Cat.No E1001) which is compatible with all SafeView™ products. Additionally, any imaging system with UV, Blue Light and/or LED should be sufficient.

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