Safe-Green™
| Cat. No. | G108-G |
| Name | Safe-Green™ |
| Unit | 1.0 ml |
| Category | Gel Documentation |
| Description |
abm's Safe-Green™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis. Features:
|
| Safeview Series | Loading Dye |
| LED Viewer Compatibility | Yes |
| Stain Color | Green |
| Application |
Safe detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels. |
| Shipping Conditions |
Shipped on blue ice packs. |
| Storage Condition |
Store at 4°C for up to 2 years. |
| Note |
Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide). |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G108-G |
| Can SafeView products be used in replacement of ethidium bromide? | |
|
All SafeView™ products (Safe-Green™, Safe-Red™, SafeView™ Classic, Safe-Red™ Gel) can be used as a complete replacement for your ethidium bromide workflow. |
| How do SafeView products work and why are they not carcinogenic? | |
|
SafeView™ products contain fluorescent compounds that have a strong affinity to nucleic acids. Once bound to a nucleic acid, the compound fluoresces under specific wavelength of light which can then be visualized using a standard UV/Blue light imager. There may be some unknown effects of SafeView™ products that have not been documented in literature but these would also apply to equivalent popular products such as SYBRSafe. However, SafeView™ products are not as carcinogenic as ethidium bromide. |
| How do I use SafeView products? | |
|
Safe-Red™ and Safe-Green™ are supplied in a 6X loading dye format: mix samples and DNA marker with Safe-Red™ or Safe-Green™ at a 1:5 (dye : sample) dilution ratio, and load onto an unstained gel. |
| Can SafeView products differentiate between double stranded and single stranded nucleic acids? | |
|
No, our SafeView products will bind to both double-stranded and single-stranded nucleic acids, albeit with lesser efficiency for single-stranded nucleic acid species.
|
| At what temperature do I store SafeView products? | |
|
SafeView Classic™, Safe-Red™ and Safe-Red™ Gel should be stored at 18-25°C. Safe-Green™ should be stored at 4°C. |
| How sensitive are SafeView products? | |
|
Safe-Green™: 0.2-0.6 ng DNA per band |
| Can SafeView products be used to post-stain gels? | |
|
Yes, SafeView™ Classic and Safe-Red™ Gel can both be used for either pre-cast or post-stain gels.
|
| What is the functional pH range for Safe-Green? | |
|
Safe-Green™ functions optimally between pH 7 to 9.
|
| Do I need to add any loading dye to my samples prior to running them on a SafeView agarose gel? | |
|
If using Safe-Green™ or Safe-Red™, you will not need to add any additional loading dyes to your samples. If using SafeView™ Classic or Safe-Red™ Gel, you will need to add an appropriate DNA loading dye to your samples in order for samples to properly settle and stay in the wells during electrophoresis. |
| We notice band shifting of our DNA fragments when using Safe-Green. Are there any recommendations that you can give us to minimize this band shifting? | |
|
Band shifting to a certain degree is unavoidable for any nucleic acid stain as most of these fluorescent compounds are large, positively charged molecules. Loading dye format nucleic acid stains, while convenient, may also suffer from more prominent band shifting due to the stain binding and migrating with the sample simultaneously during electrophoresis. Post-staining, while takes longer to complete, may be the optimal choice of nucleic acid stain strategy if band shifting issue is a priority. We have observed band shifting with Safe-Green™ as this issue has been documented in the literature to be an inherent property of the specific fluorescent compound. Alternatively we offer SafeView™ Classic which we have observed to exhibit no band shifting issues. |
| I cannot visualize 100bp and 200bp DNA bands on a 1% SafeView agarose gel. What should I do? | |
|
It is often difficult to detect smaller 100bp and 200bp bands on a 1% gel especially if samples are of low concentration. We recommend visualizing smaller fragments on a higher concentration gel - ideally 2% agarose.
|
| Can I use SafeView products to visualize nucleic acids on a polyacrylamide gel? | |
|
SafeView™ products are designed for use with agarose gels, and are not optimized for polyacrylamide gels. However, SafeView™ Classic and Safe-Red™ Gel can both visualize nucleic acids on polyacrylamide gels (Acrylamide, TBE, APS, TEMED) by post staining method.
|
| Can SafeView products be stored at -20°C? | |
|
No, please store Safe-Green™ at 4°C and SafeView™ Classic, Safe-Red™ and Safe-Red™ Gel at 18-25°C. |
| What is the concentration of SafeView products? | |
|
SafeView™ Classic and Safe-Red™ Gel are supplied at a 10,000X concentration. Safe-Green™ and Safe-Red™ are supplied at a 6X concentration.
|
| Would adding Safe-Green to the DNA ladder interfere with the migration rate of the loading dye of the ladder? | |
|
No, adding Safe-Green™ to the DNA ladder will not interfere with the DNA ladder's loading dye. You still need to add Safe-Green™ to the DNA ladder at the same ratio. To be more technically correct, Safe-Green™, and all other EB alternative DNA binding dyes, alter the migration pattern of DNA on agarose gel by shifting DNA to a larger molecular size; it is just simply because the overall molecular size of a particular DNA band increases as more dye is binding onto it. This is why we recommend mixing the DNA ladder and Safe-Green™ at the exact same 1:6 ratio, so that all samples on the gel will be subjected to the same concentration of Safe-Green™ and thus have the same shift.
|
| Can Safe-Green penetrate the nucleus? | |
|
Yes, our Safe-Green™ stain indeed has the ability to penetrate the nucleus.
|
| What color is the tracking dye of Safe-Green and Safe-Red during electrophoresis? | |
|
Safe-Green™ and Safe-Red™ both contain a dark blue tracking dye that migrates at ~300bp on a 1% agarose gel. Safe-Red™ contains an additional light blue tracking dye that migrates at ~4000bp on a 1% agarose gel.
|
| Can I perform gel extraction and subsequent cloning using SafeView products? | |
|
We recommend using SafeView™ Classic or Safe-Red™ Gel which can both be used for gel extraction of DNA fragments destined for cloning. We have observed no decrease in cloning efficiency in comparison to ethidium bromide.
|
| What type of device or imaging system should be used to visualize SafeView agarose gels? | |
|
We recommend using abm’s SafeViewER™ Imager (Cat.No E1001) which is compatible with all SafeView™ products. Additionally, any imaging system with UV, Blue Light and/or LED should be sufficient.
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