2kb Opti-DNA Marker

Cat. No.

G249

Unit

500 μl/100 loads

Price

$60.00

Cat. No.

G249

Name

2kb Opti-DNA Marker

Category

DNA & Protein Ladders

Description

PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 8 bands, ranging from 100 pb – 5 kb, for molecular weight standards in agarose gel electrophoresis. The 750 base pair band has increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.

Specifications
Number of Bands	8
DNA Range	100 bp - 5 kb
Suggested Load	Mix gently by pipetting up and down a few times, then directly load 5 μl into the well of the gel.

Application

For approximating DNA size and amount on agarose gels.

Storage Condition

Store @ 4°C for up to 3 months or -20°C for up to 24 months.

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G249

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MSDS

2kb Opti-DNA Marker

	How much of the should I load onto the gel?
	It is recommended to gently mix the ladder by pipetting up and down a few times, then load 5 μl directly into the well of the gel.

	Can I estimate the DNA mass in my sample using this ladder?
	Yes. The DNA ladder includes approximated mass values for each band (based on a 5 μl load), allowing you to estimate the mass of DNA in samples with comparable intensity and size.

	Which bands have increased intensity and why?
	The 750 bp band is of increased intensity, making it ideal as a quick visual reference point when interpreting gel results.