2nd Generation Packaging Mix

Cat. No.

LV003

Unit

200 µl

Price

$293.00

Cat. No.

LV003

Name

2nd Generation Packaging Mix

Unit

200 µl

Description

abm’s Second Generation lentivirus packaging plasmid mix offers a high-safety, high-efficiency solution for lentivirus production. This system requires three plasmids for viral particle production:

1) Transfer Plasmid containing your gene of interest.
2) Packaging Plasmid containing structural proteins Gag, Pol, Tat, Rev.
3) Envelope Plasmid containing transmembrane glycoprotein VSV-G.

The ready-to-use 2nd Generation Packaging Mix combines optimized Packaging and Envelope Plasmids for high viral titers, enhanced biosafety and reliable gene delivery performance.

Product Mix Component	Vector Map
pLenti-P2A	Click to view
pLenti-P2B	Click to view

Applications

Storage Condition

-20°C or below.

Promotions

Bundle up, save more! These cost-saving bundles combine our best-selling Lentivirus Packaging Mix with reagents for transfection, virus titration and transduction!

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV003

Datasheet

LV003 Datasheet

Search CoA here

Supporting Protocol

Other

	How do I convert a generation 3 lentiviral vector into a vector that can be used by generation 2 packaging system?
	All second generation packaging systems can package both second and third generation lentiviral expression vectors.

	Is it possible to make our own plasmid preparations from the packaging mix and then use them in transfections?
	Unfortunately it is not possible to amplify these plasmids as they are provided in a pre-made mixture.

	Can I use 2nd Generation Packaging System for generation of lentivirus for pLKO.1 ShRNA knockdown vectors?
	If you are using lentiviral vectors that are compatible with a second generation packaging system then it should be fine, however, we have not tested this in house.

	Which system should I use to package my TAT dependent vector?
	TAT dependent vectors will need to be packaged using a 2nd generation system (abm Cat LV003). 3rd generation packaging systems (abm Cat. LV053) will not be compatible with this type of vector.

	What is the concentration of your packaging mixes?
	0.5ug/ul

	What is the titer for viruses made by Cat# LV003 with 293T cells?
	For both our 2nd or 3rd generation packaging mixes, the crude viral supernatant (i.e. not concentrated nor purified) titer will be around 10^6IU/ml.

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