Ultra-Pure Lentivirus Purification Kit
| Cat. No. | LV998 |
| Name | Ultra-Pure Lentivirus Purification Kit |
| Unit | 1 kit |
| Description |
Recombinant lentivirus is widely used for cell transduction and is typically produced via transient transfection, yielding viral supernatants around 10⁶ cfu/ml—sufficient for most in vitro applications. However, higher purity and titres are needed for enhanced transduction efficiency and in vivo studies. Crude supernatants contain contaminants, making purification essential. Traditional ultracentrifugation is effective but requires specialized equipment, is technically demanding, and may compromise viral integrity. To overcome these limitations, abm developed the Ultra-Pure Lentivirus Purification Kit, featuring a proprietary Lentivirus binding matrix and ion-exchange buffers for efficient, high-yield purification with minimal damage to viral particles. |
| Storage Condition |
Store at room temperature |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV998 |
| What is the percentage loss from the purification process? | |
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On average, we found that our kit can recover 80-90% of the initial viral input, provided that the filters are not oversaturated. We found that on average, one application from this kit can process up to 10^11 viral particles in total.
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| Will this purification system remove plasmid DNA, which has been carried over from the transfection process? | |
|
No it will not, but you can add in a Benzonase treatment before Step B2 (product brochure: page 6 of 8) .
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- George, R et al. "A SHORT INTERFERING RNA MOLECULAR BEACON FOR THE ATTENUATION OF MYCOBACTERIAL INFECTION" American Journal of Biochemistry and Biotechnology 10:40-49 (2014). DOI: 10.3844/ajbbsp.2014.40.49. Application: Viral Particles Concentration.
- Liu, S., Zhang, Z., Li, L., Yao, L., Ma, Z., & Li, J. (2022). ADAM10 and γ‐secretase‐dependent cleavage of the transmembrane protein PTPRT attenuates neurodegeneration in the mouse model of Alzheimer’s disease. The FASEB Journal, 37(2). Portico. https://doi.org/10.1096/fj.202201396r