GFP Control Lentivector (Puro)
eGFP CRISPR
eGFP Lentiviral
eGFP AAV
eGFP Adenovirus
eGFP ORF
eGFP siRNA
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Product Name
GFP Control Lentivector (Puro) -
Unit
1.0 µg -
Description
A reporter gene (or reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in cell culture, animals or plants. Commonly used reporter genes that offer visually identifiable characteristics usually involve fluorescent (GFP) or luminescent (Luciferase) proteins. Application of reporter genes in vitro and in vivo have greatly advanced life science research and assay develoment. Reporter genes driven by different promoters allow accurate evaluation of promoter strength in vitro and in vivo. Over the years scientists at ABM have developed the most comprehensive line of reporter lentiviral vectors for all your research applications.
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Gene Name
eGFP -
System
lentivector -
Promoter
CMV -
Insert Size
717 bp -
Vector Size
8934 bp -
QC
Restriction Enzyme Digest and Sanger Sequencing -
Disclaimer
Print/Download Datasheet
Publishing research using LV008? Please let us know so that we can cite the reference in this datasheet.
LV008 has not been cited in any literature.
ABM community
Verified customer
Asked on Feb 28 2025
Answer
abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
ABM Scientific Support
Answered on Feb 28 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The concentration must be optimized for each cell type. Typical selection amounts are around 0.1 - 0.5µg per ml.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
ABM Scientific Support
Answered on Mar 24 2025
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Current vector selected:
GFP Control Lentivector (Puro)
Cat. No.
LV008
Plasmid DNA Services
Midiprep Plasmid Amplification
10-20 µg
$75.00
Maxiprep Plasmid Amplification
80-100 µg
$150.00
Bacterial Agar Stab
1 stab
$40.00
Virus Packaging Services
Lentivirus packaging at 107 IU/ml Titer
4 x 500 µl
$149.00
Lentivirus packaging at 108 IU/ml Titer
4 x 250 µl
$399.00
Lentivirus packaging at 109 IU/ml Titer
10 x 100 µl
$699.00
Lentivirus packaging at 1010 IU/ml Titer
10 x 100 µl
$999.00
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200 µl
$293.00
3rd Generation Packaging System Mix
LV053
200 µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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×
Current vector selected:
GFP Control Lentivector (Puro)
Cat. No.
LV008
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200 µl
$293.00
3rd Generation Packaging System Mix
LV053
200 µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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