Cas9 Nickase Adenovirus
| Cat. No. | K007 |
| Name | Cas9 Nickase Adenovirus |
| Unit | 1.0 ml |
| Description |
Adenovirus expressing Cas9 Nickase (D10A) with N-terminal SV40 NLS. This modified version of the Cas9 protein introduces single-strand breaks (nicks) in DNA instead of double-strand breaks, reducing off-target effects in genome editing. |
| Applications |
CRISPR Cas9 gene editing. |
| Titer | 106 pfu/mL |
| Storage Condition | Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K007 |
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| Is this Cas9-nickase codon-optimized for a specific cell type? | |
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Codon optimized for human, but not cell or tissue type specific.
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| Does this Cas9 nickase Cat# K007 contain any selection marker (fluorescent protein or mammalian toxin resistance like puromycin)? | |
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The Cas 9 nickase adenovirus does not contain a mammalian selection marker.
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| Is there any tag linked to the enzyme? | |
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There is no tag attached to the Cas9. This would be very difficult due to the large insert size.
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