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Cas9 Nuclease GFP NLS Protein

Cat. No.

K048

Unit

9.5 µg (50 pmol/50 µL)

Price

$57.50

Cat. No.

K048

Name

Cas9 Nuclease GFP NLS Protein

Unit

9.5 µg (50 pmol/50 µL)

Category

Cas Proteins & CRISPR Screening

Description

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.

The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).

The Cas9 from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The fusion of Cas9 Nuclease NLS to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 Nuclease-GFP can also be used for FACS applications and screening. Cas9 Nuclease-GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. Our Cas9-GFP proteins have the Enhanced green fluorescent protein (eGFP).

abm offers a variety of Cas9 proteins. View our whole collection here.

Cas Type

Nuclease

Cas Origin

spCas9

Cas Protein Marker

GFP

Concentration

1000 nM, 188 µg/ml

Format General

Enzyme supplied with 10X Reaction Buffer

Storage Buffer

10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.

Storage Condition

Store all components at -20°C

Caution

This product is distributed for laboratory research only. Not for diagnostic use.

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K048

Datasheet

Cas9 Nuclease GFP NLS Protein Datasheet

Search CoA here

MSDS

Cas9 Proteins MSDS

	Does K048 contain a HIS-tag?
	Yes, it does contain a His-tag.

	What is the source of this enzyme?
	The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.

Rahimi, H., Zaboli, K. A., Thekkiniath, J., Mousavi, S. H., Johari, B., Hashemi, M. R., Nosrati, H., Goldschneider, D., Bernet, A., Danafar, H., & Kaboli, S. (2022). BSA-PEI Nanoparticle Mediated Efficient Delivery of CRISPR/Cas9 into MDA-MB-231 Cells. Molecular Biotechnology, 64(12), 1376–1387. https://doi.org/10.1007/s12033-022-00514-z
Lee, N., Park, J., Kim, J. E., Shin, J. Y., Min, K., & Son, H. (2022). Genome editing using preassembled CRISPR-Cas9 ribonucleoprotein complexes in Fusarium graminearum. PloS one, 17(6), e0268855. https://doi.org/10.1111/pre.12472
Ichihara, K., Yamazaki, T., & Kawano, S. (2022). Genome editing using a DNA‐free clustered regularly interspaced short palindromic repeats‐Cas9 system in green seaweed Ulva prolifera. Phycological Research, 70(1), 50-56. https://doi.org/10.1111/pre.12472

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