Column-Pure RNA Miniprep Kit

Cat. No.

D518

Unit

50 preparations

Price

$307.00

Cat. No.

D518

Name

Column-Pure RNA Miniprep Kit

Unit

50 preparations

Description

abm’s Column-Pure RNA Miniprep Kit is a fast and efficient method for the isolation and purification of total RNA from mammalian cells, tissues, yeast and bacteria. The silica spin column technology allows for rapid recovery of high quality RNA that is ready for downstream applications such as RT-PCR, qPCR, cDNA libraries and Northern blotting.

Key Features:

Simple, rapid 25 min protocol
Extract high yield & purity RNA from a variety of sample types
On-column genomic DNA removal
High binding capacity up to 200 µg RNA
Compatible with lower input cell numbers (e.g. 100-1x10⁷ HEK293T cells)
No phenol/chloroform, LiCl or alcohol precipitation required

Customer Testimonial

"abm's Column-Pure RNA Miniprep Kit was incredibly easy to use, thanks to the clear and straightforward instructions. The protocol also required much shorter centrifugation steps compared to other kits I've used. Despite starting with a low number of cells, I obtained RNA concentrations above 600 ng/µl with excellent purity; both 260/280 and 260/230 ratios were consistently around 2.0. qPCR results confirmed there was no genomic DNA contamination. I truly enjoyed using this kit and highly recommend it!"

-Bahareh, BC Children's Research Institute

Note

Additional Materials Required:

Material	Required For
14.3M β-mercaptoethanol	All Protocols
95-100% Ethanol	All Protocols
70% Ethanol	All Protocols
Zymolyase or Lyticase & Reaction Buffer	Yeast Protocol
Lysozyme	Bacteria Protocol

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. D518

Datasheet

D518 Datasheet

Search CoA here

MSDS

D518 MSDS

There are no FAQs for this product yet!

Liu, Y., Betori, R. C., Pagacz, J., Frost, G. B., Efimova, E. V., Wu, D., Wolfgeher, D. J., Bryan, T. M., Cohen, S. B., Scheidt, K. A., & Kron, S. J. (2022). Targeting telomerase reverse transcriptase with the covalent inhibitor NU-1 confers immunogenic radiation sensitization. Cell Chemical Biology, 29(10), 1517-1531.e7. https://doi.org/10.1016/j.chembiol.2022.09.002

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